MYC tag Polyclonal antibody

MYC tag Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16286-1-AP

Synonyms

EQKLISEEDL, myc, MYC tag, myc-tag


"MYC tag Antibodies" Comparison

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Applications:
  • Western Blot (WB) analysis of Recombinant protein using MYC tag Polyclonal antibody (16286-1-AP)

    WB analysis of Recombinant protein using 16286-1-AP

    Recombinant protein were subjected to SDS PAGE followed by western blot with 16286-1-AP (MYC-tag antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Transfected HEK-293 cells using MYC tag Polyclonal antibody (16286-1-AP)

    IP experiment of Transfected HEK-293 using 16286-1-AP

    IP Result of anti-MYC-tag (IP:16286-1-AP, 4ug; Detection:16286-1-AP 1:1000) with Transfected HEK-293 cells lysate 300ug.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using MYC tag Polyclonal antibody (16286-1-AP)

    IF Staining of Transfected HEK-293 using 16286-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using MYC tag antibody (16286-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using MYC tag Polyclonal antibody (16286-1-AP)

    IF Staining of Transfected HEK-293 using 16286-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using MYC tag antibody (16286-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MYC tag Polyclonal antibody (16286-1-AP)

    IF Staining of MCF-7 using 16286-1-AP

    Immunofluorescent analysis of MCF-7 cells, using MYC-tag antibody 16286-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).


Tested Applications

Positive WB detected inRecombinant protein protein, Recombinant protein
Positive IP detected inTransfected HEK-293 cells
Positive IF detected inTransfected HEK-293 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 16286-1-AP is MYC tag Fusion Protein expressed in E. coli.

Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYC tag fusion protein Ag9409
Full Name MYC tag
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The myc-tag is a short synthetic polypeptide sequence derived from c-myc protein that can be added to recombinant proteins to enable isolation and study when an antibody is not available. This antibody recognizes the MYC tag EQKLISEEDL.

What is the molecular weight of myc?

1203Da: the ten amino acid myc tag sequence is EQKLISEEDL (in single letter code).

What are the applications for myc tag?

The addition of the myc tag to a particular protein can be useful when an antibody is not available to the protein of interest. Using recombinant DNA technology, the myc tag can be fused to the protein and then an antibody against the myc tag can be used to probe (PMID: 24490106). This is a reliable method and can be used in a number of different techniques, including purification using chromatography, tracking the protein in localization studies using immunofluorescence, or quantifying levels using Western Blot (PMID: 24490106). 

What is the structure of myc tag?

The c-myc gene from which this tag is derived has a molecular weight of 49kDa, but the myc tag represents only a small portion of the C-terminus of this gene. The short polypeptide sequence can be fused to the N-terminus or the C-terminus of any protein without influencing function, although it is advised to avoid fusing it to a secretory signal. A cleavage site behind this tag is also sometimes engineered to allow removal with a specific protease.


Protocols

Product Specific Protocols
WB protocol for MYC tag antibody 16286-1-APDownload protocol
IF protocol for MYC tag antibody 16286-1-APDownload protocol
IP protocol for MYC tag antibody 16286-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB,IF

Mol Cell

Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells.

Authors - Jia Xiong
IP

Nat Struct Mol Biol

ARF GTPases activate Salmonella effector SopF to ADP-ribosylate host V-ATPase and inhibit endomembrane damage-induced autophagy.

Authors - Yue Xu
WB

Mol Cancer

Circular RNA hsa_circ_0004872 inhibits gastric cancer progression via the miR-224/Smad4/ADAR1 successive regulatory circuit.

Authors - Cunying Ma
WB

Mol Cancer

CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer.

Authors - Ya Fan
WB,IP

Mol Cancer

CHEK1 and circCHEK1_246aa evoke chromosomal instability and induce bone lesion formation in multiple myeloma.

Authors - Chunyan Gu
WB

Sci Adv

Gene therapy for tuberous sclerosis complex type 2 in a mouse model by delivery of AAV9 encoding a condensed form of tuberin.

Authors - Pike-See Cheah

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (08-03-2022)

gives a good signal in IF

  • Applications: Immunofluorescence
FH

Chahat (Verified Customer) (06-08-2022)

The myc antibody gives clear staining!

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: ES2
FH

WEI (Verified Customer) (03-08-2022)

Good antibody for detecting overexpression proteins in 293 cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: 293 cells
FH

Minze (Verified Customer) (01-11-2022)

it works well for immunofluorecence so far.

  • Applications: Western Blot, Immunofluorescence
  • Cell Tissue Type: BHK-21
FH

Phoebe (Verified Customer) (03-01-2019)

This antibody was very clean in a western blot to detect Myc-tagged pol2 in S cerevisiae whole cell extract and chromatin fractionation samples.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: S cerevisiae