MYH6 Antibody

MYH6 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22281-1-AP

Synonyms

alpha MHC, ASD3, MYH6, MYHC, MyHC alpha, MYHCA, Myosin 6, Myosin heavy chain 6



Tested Applications

Positive WB detected inrat heart tissue
Positive IHC detected inhuman heart tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

22281-1-AP targets MYH6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name myosin, heavy chain 6, cardiac muscle, alpha
Calculated molecular weight 224 kDa
Observed molecular weight 200-220 kDa
GenBank accession number NM_002471
Gene symbol MYH6
Gene ID (NCBI) 4624
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYH6 encodes the alpha-cardiac myosin heavy chain (aMHC) which is a major contractile protein exclusively expressed in the myocardium of the heart. Expression of MYH6 is down-regulated in cardiac myopathy and failing hearts. Defects in MYH6 lead to congenital heart disease (CHD), and a spectrum of phenotypes ranging from hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM). (15737621, 20656787)

Protocols

Product Specific Protocols
WB protocol for MYH6 antibody 22281-1-APDownload protocol
IHC protocol for MYH6 antibody 22281-1-APDownload protocol
IF protocol for MYH6 antibody 22281-1-APDownload protocol
IP protocol for MYH6 antibody 22281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Br J Pharmacol

Hydrogen Sulfide Regulates Muscle RING Finger-1 Protein S-Sulfhydration at Cys44 to Prevent Cardiac Structural Damage in Diabetic Cardiomyopathy.

Authors - Xiaojiao Sun
ratWB

Front Public Health

Examination of the Effect of a 50-Hz Electromagnetic Field at 500 μT on Parameters Related With the Cardiovascular System in Rats.

Authors - Yemao Zhang
mouseIHC

BMC Genet

Skeletal muscle in aged mice reveals extensive transformation of muscle gene expression.

Authors - I-Hsuan Lin
MOUSEWB,IF

Cell Physiol Biochem

Overexpression of Cardiac-Specific Kinase TNNI3K Promotes Mouse Embryonic Stem Cells Differentiation into Cardiomyocytes.

Authors - Yin Wang
humanIHC

Sci Rep

Region of Interest analysis using mass spectrometry imaging of mitochondrial and sarcomeric proteins in acute cardiac infarction tissue.

Authors - Yuka Yajima
mouse

J Pathol

Doxorubicin-induced cardiotoxicity involves IFNγ-mediated metabolic reprogramming in cardiomyocytes.

Authors - Chen Ni