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  • Western Blot (WB) analysis of L02 cells using MYLK3 Recombinant antibody (83673-3-RR)

    WB analysis of L02 using 83673-3-RR

    L02 cells were subjected to SDS PAGE followed by western blot with 83673-3-RR (MYLK3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using MYLK3 Recombinant antibody (83673-3-RR)

    WB analysis of human heart using 83673-3-RR

    human heart tissue were subjected to SDS PAGE followed by western blot with 83673-3-RR (MYLK3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83673-3-RR

    Affinity and Kinetic Characterization of 83673-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83673-3-RR against Human MYLK3 were performed. The affinity constant is below 1 pM.

MYLK3 Recombinant monoclonal antibody

MYLK3 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 83673-3-RR
Clone No.240762G7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

MLCK, EC:2.7.11.18, Cardiac-MyBP-C-associated Ca/CaM kinase, Cardiac-MLCK, caMLCK

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inL02 cells, human heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83673-3-RR targets MYLK3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag16137

Product name: Recombinant human MYLK3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC109097

Sequence: MDTKLNMLNEKVDQLLHFQEDVTEKLQSMCRDMGHLERGLHRLEASRAPGPGGADGVPHIDTQAGWPEVLELVRAMQQDAAQHGARLEALFRMVAAVDRAIALVGATFQKSKVADFLMQGRVPWRRGSPGDSPEENKERVEEEGGKPKHVLSTSGLQSDAREPGEESQKADVLEGTAERLPPIRASGLGADPAQAVVSPGQGDGVPGPAQAFPGHLPLPTKVEAKAPETPSENLRTGLELAPAPGRVNVVSPSLEVAPGAGQGASSSRPDPEPLEEGTRLTPGPGPQCPGPPGLPAQARATHSGGETPPRISIHIQEMDTPGEMLMTGRGSLGPTLTTEAPAAAQPGKQG

Predict reactive species
Full Name myosin light chain kinase 3
Calculated Molecular Weight 819 aa, 88 kDa
Observed Molecular Weight95 kDa
GenBank Accession NumberBC109097
Gene Symbol MYLK3
Gene ID (NCBI) 91807
RRIDAB_3671278
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ32MK0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cardiac myosin light chain kinase (cMLCK) encoded by the myosin light chain kinase 3 (MYLK3) phosphorylates cardiac myosin light chain 2 (MLC2, encoded by MYL2) and facilitates actin-myosin interactions that enhance contractile force in the animal heart (PMID: 29235529).

Protocols

Product Specific Protocols
WB protocol for MYLK3 antibody 83673-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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