Tested Applications
| Positive WB detected in | HeLa cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, A549 cells, RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85927-1-RR targets MYSM1-Specific in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | Myb-like, SWIRM and MPN domains 1 |
| Calculated Molecular Weight | 95 kDa |
| Observed Molecular Weight | 81 kDa |
| GenBank Accession Number | NM_001085487 |
| Gene Symbol | MYSM1 |
| Gene ID (NCBI) | 114803 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q5VVJ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYSM1, also named as KIAA1915, belongs to the peptidase M67A family and MYSM1 subfamily. MYSM1 is a metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. MYSM1 acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation. It catalyzes the reaction: Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol. MYSM1 is a marker of cancer. The antibody is specific to MYSM1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYSM1-Specific antibody 85927-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
