Mouse IL-1 alpha ELISA Kit

Sensitivity

2.7 pg/mL

Range

7.8-500 pg/mL

Reactivity

Mouse

Cat no : KE10024

Synonyms

IL 1, Il 1a, IL1, IL-1, IL1 alpha, IL-1 alpha, Il1a, IL1alpha, IL-1alpha, interleukin 1 alpha, Interleukin-1 alpha



Product Information

KE10024 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL1-alpha ELISA kit is to be used to detect and quantify protein levels of endogenous IL1-alpha. The assay recognizes mouse IL1-alpha. An antibody specific for IL1-alpha has been pre-coated onto the microwells. The IL1-alpha protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL1-alpha is added to detect the captured IL1-alpha protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Mouse IL-1 alpha ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 2.7 pg/mL
Range 7.8-500 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Mouse serum87%77%-103%
Cell culture supernatants101%94%-118%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12033.92.16.1
22095.03.53.7
320388.715.03.9

InterAssay

Samplenmean ( pg/mL)SDCV%
12437.03.69.6
224117.67.36.3
324504.945.89.1

Background Information

Interleukin-1α (Il-1α) is a protein that plays a crucial role in the regulation of a number of key cellular processes. Il-1α is a cytokine and a potent mediator of body’s response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that Il-1α also has a role in wound healing, rheumatoid arthritis, Alzheimer’s disease and tumor growth. Il-1α functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. Il-1α was found to affect the cell cycle of osteosarcoma cell to reduce cell growth.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms IL 1, Il 1a, IL1, IL-1, IL1 alpha, IL-1 alpha, Il1a, IL1alpha, IL-1alpha, interleukin 1 alpha, Interleukin-1 alpha

Publications

SpeciesSample TypeTitle
mousecell culture supernatants

Front Immunol

Inhibitory Effects of the Lactobacillus rhamnosus GG Effector Protein HM0539 on Inflammatory Response Through the TLR4/MyD88/NF-кB Axis.

Authors - Yubin Li