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NANOG Polyclonal antibody

NANOG Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14295-1-AP

Synonyms

hNanog, Homeobox protein NANOG, NANOG, Nanog homeobox



Tested Applications

Positive WB detected inrat brain tissue, human brain tissue, mouse brain tissue
Positive IF detected inhuman embronic stem cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14295-1-AP targets NANOG in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, marmoset, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NANOG fusion protein Ag5645
Full Name Nanog homeobox
Calculated molecular weight 35 kDa
Observed molecular weight 43-48 kDa
GenBank accession numberBC160187
Gene symbol NANOG
Gene ID (NCBI) 79923
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells. There are two kinds of variants could recognized by NANOG, one is normal form (~39kd), the other is post-translation modified form (~48kd) (21136380 ). Nanog exists two isoforms with molecular weight 34.4 kDa and 31.9 kDa. (PMID: 21969378)

Protocols

Product Specific Protocols
WB protocol for NANOG antibody 14295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EBioMedicine

Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.

Authors - Binghua Li
humanWB

Int J Food Sci Nutr

Sulforaphane inhibits gastric cancer stem cells via suppressing sonic hedgehog pathway.

Authors - Miaomiao Ge
mouseWB,IHC

J Exp Clin Cancer Res

Tobacco smoke induced hepatic cancer stem cell-like properties through IL-33/p38 pathway.

Authors - Chunfeng Xie
humanIF

Stem Cells Dev

Rapid Differentiation of Multi-Zone Ocular Cells from Human Induced Pluripotent Stem Cells and Generation of Corneal Epithelial and Endothelial Cells.

Authors - Zongyi Li
humanWB

Front Oncol

Scorpion Venom Analgesic Peptide, BmK AGAP Inhibits Stemness, and Epithelial-Mesenchymal Transition by Down-Regulating PTX3 in Breast Cancer.

Authors - Sylvanus Kampo
humanWB,IHC,IF

Cell Death Dis

Soft fibrin matrix downregulates DAB2IP to promote Nanog-dependent growth of colon tumor-repopulating cells.

Authors - Meng Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ying (Verified Customer) (08-19-2019)

very good antibody.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: mouse primary cardiomyocytes