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NCKIPSD Antibody

NCKIPSD Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11367-1-AP

Synonyms

AF3P21, Dia interacting protein 1, DIP, DIP 1, DIP1, NCKIPSD, ORF1, SH3 adapter protein SPIN90, SPIN90, VIP54, WASLBP, WISH



Tested Applications

Positive WB detected inmouse brain tissue, fetal human brain tissue, human heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11367-1-AP targets NCKIPSD in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCKIPSD fusion protein Ag1915
Full Name NCK interacting protein with SH3 domain
Calculated molecular weight 715aa,78 kDa
Observed molecular weight 80 kDa
GenBank accession number BC016052
Gene symbol NCKIPSD
Gene ID (NCBI) 51517
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NCKIPSD antibody 11367-1-APDownload protocol
IHC protocol for NCKIPSD antibody 11367-1-APDownload protocol
IP protocol for NCKIPSD antibody 11367-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Cancer Res

SPIN90 depletion and Microtubule Acetylation Mediate Stromal Fibroblast Activation in Breast Cancer Progression.

Authors - Eunae You
  • KO Validated