NDNL2 Antibody

NDNL2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27488-1-AP

Synonyms

HCA4, MAGE G1 antigen, MAGEG1, MAGEL3, Melanoma associated antigen G1, NDNL2, necdin like 2, Necdin like protein 2, NSE3, NSMCE3



Tested Applications

Positive WB detected inHepG2 cells, L02 cells, HeLa cells, human placenta tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27488-1-AP targets NDNL2 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDNL2 fusion protein Ag26663
Full Name necdin-like 2
Calculated molecular weight 34 kDa
Observed molecular weight 38 kDa
GenBank accession number BC053999
Gene symbol NDNL2
Gene ID (NCBI) 56160
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDNL2 (also known as NSMCE3 or MAGEG1) is a part of the SMC5-6 protein complex that is essential for DNA damage response and chromosome segregation (PMID: 18086888; 27427983). The gene of human NDNL2 maps to chromosome 15q13.1. Northern blot analysis detected expression of a 1.9-kb transcript in all human tissues tested, with highest expression in testis (PMID: 11782285). Missense mutations in NSMCE3 have been associated with an autosomal recessive chromosome breakage syndrome that leads to defective T and B cell function and acute respiratory distress syndrome in early childhood (PMID: 27427983).

Protocols

Product Specific Protocols
WB protocol for NDNL2 antibody 27488-1-APDownload protocol
IHC protocol for NDNL2 antibody 27488-1-APDownload protocol
IF protocol for NDNL2 antibody 27488-1-APDownload protocol
Standard Protocols
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