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  • KD/KO Validated

NDUFB10 Polyclonal antibody

NDUFB10 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15589-1-AP

Synonyms

CI PDSW, Complex I PDSW, NDUFB10, PDSW



Tested Applications

Positive WB detected inHepG2 cells, human skeletal muscle tissue, mouse liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human brain tissue, human heart tissue, human kidney tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15589-1-AP targets NDUFB10 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFB10 fusion protein Ag7922
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10, 22kDa
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC005829
Gene symbol NDUFB10
Gene ID (NCBI) 4716
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDUFB10(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10) is also named as PDSW and belongs to the complex I NDUFB10 subunit family. It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. The Complex I functions in the transfer of electrons from NADH to the respiratory chain.

Protocols

Product Specific Protocols
WB protocol for NDUFB10 antibody 15589-1-APDownload protocol
IHC protocol for NDUFB10 antibody 15589-1-APDownload protocol
IP protocol for NDUFB10 antibody 15589-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Endocrinol (Lausanne)

GIT2 Acts as a Systems-Level Coordinator of Neurometabolic Activity and Pathophysiological Aging.

Authors - Bronwen Martin
mouseWB

Aging (Albany NY)

Genomic deletion of GIT2 induces a premature age-related thymic dysfunction and systemic immune system disruption.

Authors - Sana Siddiqui
humanWB

Brain Pathol

Mitochondrial activity in the frontal cortex area 8 and angular gyrus in Parkinson's disease and Parkinson's disease with dementia.

Authors - Paula Garcia-Esparcia
humanWB

Cell Rep

A Role for Mitochondrial Translation in Promotion of Viability in K-Ras Mutant Cells.

Authors - Timothy D Martin
  • KO Validated
humanWB

Proc Natl Acad Sci U S A

Assembly factors for the membrane arm of human complex I.

Authors - Andrews Byron B
humanWB

Nat Cell Biol

LIN28 phosphorylation by MAPK/ERK couples signalling to the post-transcriptional control of pluripotency.

Authors - Kaloyan M Tsanov