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- KD/KO Validated
NDUFV1 Polyclonal antibody
NDUFV1 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC,ELISA
Conjugate
Unconjugated
Cat no : 11238-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, human brain tissue |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 20 publications below |
Product Information
11238-1-AP targets NDUFV1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, swine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NDUFV1 fusion protein Ag1764 |
| Full Name | NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa |
| Calculated molecular weight | 51 kDa |
| Observed molecular weight | 51 kDa |
| GenBank accession number | BC015645 |
| Gene symbol | NDUFV1 |
| Gene ID (NCBI) | 4723 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DUFV1(NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial) is also named as UQOR1,Complex I-51kD and belongs to the complex I 51 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. It has 2 isoforms produced by alternative splicing. Defects in NDUFV1 are a cause of Leigh syndrome (LS) and mitochondrial complex I deficiency (MT-C1D).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NDUFV1 antibody 11238-1-AP | Download protocol |
| IHC protocol for NDUFV1 antibody 11238-1-AP | Download protocol |
| IP protocol for NDUFV1 antibody 11238-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO Rep CLPP deficiency protects against metabolic syndrome but hinders adaptive thermogenesis. | ||
Front Genet Novel MT-ND Gene Variants Causing Adult-Onset Mitochondrial Disease and Isolated Complex I Deficiency. | ||
Am J Transl Res Fenofibrate-induced mitochondrial dysfunction and metabolic reprogramming reversal: the anti-tumor effects in gastric carcinoma cells mediated by the PPAR pathway. | ||
J Neurochem Complex I specific increase in superoxide formation and respiration rate by PrP-null mouse brain mitochondria. | ||
Sci Rep Effects of glucose metabolism pathways on nuclear and cytoplasmic maturation of pig oocytes.
| ||
J Cell Physiol Glucose metabolism during in vitro maturation of mouse oocytes: An study using RNA interference. |
