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  • KD/KO Validated

NDUFV2 Polyclonal antibody

NDUFV2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15301-1-AP

Synonyms

NDUFV2



Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue, rat skeletal muscle tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman prostate cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15301-1-AP targets NDUFV2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFV2 fusion protein Ag7559
Full Name NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa
Calculated molecular weight 27 kDa
Observed molecular weight 24-27 kDa
GenBank accession numberBC001632
Gene symbol NDUFV2
Gene ID (NCBI) 4729
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The NDUFV2 gene encodes the 24-kD subunit of the mitochondrial NADH:ubiquinone oxidoreductase (complex I of the respiratory chain). The protein belongs to the complex I 24 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. NDUFV2 constitutes one genetic risk factor for PD, and the mutation may well be a cause of complex I deficiency in this disease(PMID:9570948).

Protocols

Product Specific Protocols
WB protocol for NDUFV2 antibody 15301-1-APDownload protocol
IHC protocol for NDUFV2 antibody 15301-1-APDownload protocol
IF protocol for NDUFV2 antibody 15301-1-APDownload protocol
IP protocol for NDUFV2 antibody 15301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

BMC Res Notes

Subunit composition of respiratory chain complex 1 and its responses to oxygen in mitochondria from human donor livers.

Authors - S E Khorsandi
mouseWB

EMBO Rep

CLPP deficiency protects against metabolic syndrome but hinders adaptive thermogenesis.

Authors - Christina Becker
ratIF

Acta Histochem Cytochem

Increased Expression of Osteopontin in the Degenerating Striatum of Rats Treated with Mitochondrial Toxin 3-Nitropropionic Acid: A Light and Electron Microscopy Study.

Authors - Hong-Lim Kim
mouseWB, IF

Brain Res

NDUFV2 regulates neuronal migration in the developing cerebral cortex through modulation of the multipolar-bipolar transition.

Authors - Tianda Chen
  • KD Validated
humanWB

Am J Transl Res

Fenofibrate-induced mitochondrial dysfunction and metabolic reprogramming reversal: the anti-tumor effects in gastric carcinoma cells mediated by the PPAR pathway.

Authors - Lulu Chen
mouseWB

Chemosphere

Mitochondrial respiratory chain damage and mitochondrial fusion disorder are involved in liver dysfunction of fluoride-induced mice.

Authors - Hong-Wei Wang