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NDUFV2 Antibody 12 Publications

Rabbit Polyclonal| Catalog number: 15301-1-AP

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

NDUFV2 Antibody WB mouse heart tissue 15301-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 15301-1-AP (NDUFV2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours

NDUFV2 Antibody IHC human prostate cancer tissue 15301-1-AP

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15301-1-AP (NDUFV2 antibody) at dilution of 1:1000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

NDUFV2 Antibody IHC human prostate cancer tissue 15301-1-AP

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15301-1-AP (NDUFV2 antibody) at dilution of 1:1000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

NDUFV2 Antibody IF Hela cells 15301-1-AP

Immunofluorescent analysis of Hela cells, using NDUFV2 antibody 15301-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

NDUFV2 Antibody IP mouse heart tissue 15301-1-AP

IP Result of anti-NDUFV2 (IP:15301-1-AP, 3ug; Detection:15301-1-AP 1:400) with mouse heart tissue lysate 4000ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse heart tissue, rat heart tissue, rat skeletal muscle tissue
Positive IP detected in:
mouse heart tissue
Positive IHC detected in:
human prostate cancer tissue, mouse heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
Hela cells
Recommended dilution:
WB : 1:5000-1:20000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:500-1:2000
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
NDUFV2 fusion protein Ag7559
Full name:
NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa
Calculated molecular weight:
27 kDa
Observed molecular weight:
24-27 kDa
GenBank accession number:
BC001632
Gene ID (NCBI):
4729
Gene symbol
NDUFV2
Synonyms
NDUFV2

Background

The NDUFV2 gene encodes the 24-kD subunit of the mitochondrial NADH:ubiquinone oxidoreductase (complex I of the respiratory chain). The protein belongs to the complex I 24 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. NDUFV2 constitutes one genetic risk factor for PD, and the mutation may well be a cause of complex I deficiency in this disease(PMID:9570948).


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