Tested Applications
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19706-1-AP targets NEB-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | nebulin |
| Calculated Molecular Weight | 773 kDa |
| GenBank Accession Number | NM_004543 |
| Gene Symbol | NEB |
| Gene ID (NCBI) | 4703 |
| RRID | AB_10637999 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20929 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NEB, a giant muscle protein, may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. NEB binds and stabilizes F-actin. Defects in NEB are the cause of nemaline myopathy type 2 (NEM2). The antibody is specific to NEB.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NEB-Specific antibody 19706-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NEB-Specific Polyclonal antibody (Cat# 19706-1-AP) has 2 total citations. These appear in Journal of Clinical Investigation and eLife. The cited research focuses on skeletal muscle biology, specifically nemaline myopathy and sarcomere stability, involving the giant muscle protein NEBulin in human and mouse models.
| Species | Application | Title |
|---|---|---|
J Clin Invest KLHL40 deficiency destabilizes thin filament proteins and promotes nemaline myopathy. | ||
