Tested Applications
| Positive WB detected in | HepG2 cells, A549 cells, DMSO treated HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human colon cancer tissue, human kidney tissue, human pancreas cancer tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MG132 treated HepG2 cells, HepG2 cells |
This antibody is only recommended for WB detection of human samples
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16396-1-AP targets NRF2, NFE2L2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA, IHC-IF applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, monkey, chicken, sheep, goat, yellow catfish, seiurus aurocapilla, ducks |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NRF2, NFE2L2 fusion protein Ag9489 Predict reactive species |
| Full Name | nuclear factor (erythroid-derived 2)-like 2 |
| Calculated Molecular Weight | 605 aa, 68 kDa |
| Observed Molecular Weight | 110 kDa, 68 kDa |
| GenBank Accession Number | BC011558 |
| Gene Symbol | NRF2 |
| Gene ID (NCBI) | 4780 |
| RRID | AB_2782956 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16236 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NRF2, NFE2L2 antibody 16396-1-AP | Download protocol |
| IHC protocol for NRF2, NFE2L2 antibody 16396-1-AP | Download protocol |
| IF protocol for NRF2, NFE2L2 antibody 16396-1-AP | Download protocol |
| IP protocol for NRF2, NFE2L2 antibody 16396-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Gastroenterology Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis | ||
Cancer Cell iASPP Is an Antioxidative Factor and Drives Cancer Growth and Drug Resistance by Competing with Nrf2 for Keap1 Binding.
| ||
Cell Metab Transsulfuration Activity Can Support Cell Growth upon Extracellular Cysteine Limitation. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH PK (Verified Customer) (08-15-2024) | Excellent
 |
FH SD (Verified Customer) (07-05-2024) | Excellent
 |
FH Guillermo (Verified Customer) (09-03-2022) | Very good product, great spots.
|
FH balawant (Verified Customer) (08-06-2022) | for westerblotting I have used this Antibody. this antibody is excellent and I will recommend if you are working with this protein
|
FH Georgina (Verified Customer) (07-04-2022) | This is a good antibody - it gives some background signal but the band of interest is clear. Sometimes need to use a higher concentration than recommended for cells with lower expression of NRF2.
|
FH Iram (Verified Customer) (03-11-2022) | Gave good bands around 100 kda in colon cancer cell lysates
|
FH Giovanni (Verified Customer) (01-11-2022) | the antiboby worked very well at dilution 1:1000 in WB using Htr8/SVneo cell line lysates. It gave a specific band at around 100 kDa with very low background.
 |
FH Xiaoping (Verified Customer) (10-14-2021) | Gave good bands around 100 kda in renal cell lysates
|
FH Razia Sultana (Verified Customer) (08-20-2020) | The antibody gives good band at around 100KD(100 KD in HK2 cells and 90KD in kidney tissue lysate) along with some non specific bands at around 40-50KD. The trial size gave a band at 130KD which is unusual and lot of non specific bands,but the full size antibody is good. We are satisfied with the purchase
|
FH JIM (Verified Customer) (03-26-2019) | Strong Nrf2 signal can be observed in macrophages treated with LPS and D3T by western blots.
|
