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NRF2, NFE2L2 Antibody 113 Publications

Rabbit Polyclonal| Catalog number: 16396-1-AP

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Con: 70 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

NRF2, NFE2L2 Antibody WB HeLa cells 16396-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 16396-1-AP (NRF2, NFE2L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

NRF2, NFE2L2 Antibody IP mouse kidney tissue 16396-1-AP

IP Result of anti-NRF2, NFE2L2 (IP:16396-1-AP, 3ug; Detection:16396-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

NRF2, NFE2L2 Antibody IHC human ovary tumor tissue 16396-1-AP

Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 16396-1-AP( NRF2, NFE2L2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

NRF2, NFE2L2 Antibody FC MCF-7 cells 16396-1-AP

1X10^6 MCF-7 cells were stained with 0.2ug NRF2, NFE2L2 antibody (16396-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat, ostrich chick, pig
Positive WB detected in:
HeLa cells, DMSO treated HeLa cells
Positive IP detected in:
mouse kidney tissue
Positive IHC detected in:
human ovary tumor tissue, human breast cancer tissue, human colon cancer tissue, human kidney tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in:
MCF-7 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
NRF2, NFE2L2 fusion protein Ag9489
Full name:
nuclear factor (erythroid-derived 2)-like 2
Calculated molecular weight:
605aa,68 kDa
Observed molecular weight:
110, 68 kDa
GenBank accession number:
BC011558
Gene ID (NCBI):
4780
Gene symbol
NFE2L2
Synonyms
HEBP1, NF E2 related factor 2, NFE2 related factor 2, NFE2L2, NRF2

Background

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes. The expression of NRF2 may be induced by oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection of chemotherapy in NSCLC. The calculated molecular weight of the Nrf2 protein is about 67 kDa, but Nrf2 protein can form a heterodimer with MafG in the cell nucleus, and molecular weight of heterodimer is 110 kDa.


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