NOL3 Polyclonal antibody

NOL3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10846-2-AP

Synonyms

Apoptosis repressor with CARD, ARC, CARD2, MYP, NOL3, NOP, NOP30, Nucleolar protein 3, Nucleolar protein of 30 kDa



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10846-2-AP targets NOL3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOL3 fusion protein Ag1306
Full Name nucleolar protein 3 (apoptosis repressor with CARD domain)
Calculated molecular weight 24 aa, 3 kDa
Observed molecular weight 30-33 kDa
GenBank accession numberBC012798
Gene symbol NOL3
Gene ID (NCBI) 8996
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NOL3 antibody 10846-2-APDownload protocol
IHC protocol for NOL3 antibody 10846-2-APDownload protocol
IF protocol for NOL3 antibody 10846-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Cycle

Long noncoding RNA PCAT6 inhibits colon cancer cell apoptosis by regulating anti-apoptotic protein ARC expression via EZH2.

Authors - Weimei Huang
mouseWB

Pharmacol Biochem Behav

Involvement of microglial cells in the antinociceptive effects of metamizol in a mouse model of neuropathic pain.

Authors - R Zajaczkowska
mouseIF

Hum Genet

MS/MS in silico subtraction-based proteomic profiling as an approach to facilitate disease gene discovery: application to lens development and cataract.

Authors - Sandeep Aryal
ratWB

Cell Death Dis

MicroRNA-30d regulates cardiomyocyte pyroptosis by directly targeting foxo3a in diabetic cardiomyopathy.

Authors - X Li