Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10846-2-AP targets NOL3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1306 Product name: Recombinant human NOL3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-208 aa of BC012798 Sequence: MGNAQERPSETIDRERKRLVETLQADSGLLLDALLARGVLTGPEYEALDALPDAERRVRRLLLLVQGKGEAACQELLRCAQRTAGAPDPAWDWQHVGPGYRDRSYDPPCPGHWTPEAPGSGTTCPGLPRASDPDEAGGPEGSEAVQSGTPEEPEPELEAEASKEAEPEPEPEPELEPEAEAEPEPELEPEPDPEPEPDFEERDESEDS Predict reactive species |
| Full Name | nucleolar protein 3 (apoptosis repressor with CARD domain) |
| Calculated Molecular Weight | 24 aa, 3 kDa |
| Observed Molecular Weight | 30-33 kDa |
| GenBank Accession Number | BC012798 |
| Gene Symbol | NOL3 |
| Gene ID (NCBI) | 8996 |
| RRID | AB_2151850 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60936 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NOL3 antibody 10846-2-AP | Download protocol |
| IHC protocol for NOL3 antibody 10846-2-AP | Download protocol |
| WB protocol for NOL3 antibody 10846-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-ARC antibody (Cat# 10846-2-AP) has 11 total citations published in journals including Cell Death & Disease, JCI Insight, Drug Delivery, BBA – Molecular Basis of Disease, Cell Cycle, Journal of Orthopaedic Surgery and Research, Human Genetics, Frontiers in Genetics, World Journal of Surgical Oncology, Pharmacology Biochemistry and Behavior, and bioRxiv. Research fields span cardiomyopathy, hepatocellular carcinoma, colon cancer, necroptosis, mitophagy, cataract, bladder cancer, and neuropathic pain.
| Species | Application | Title |
|---|---|---|
Cell Death Dis MicroRNA-30d regulates cardiomyocyte pyroptosis by directly targeting foxo3a in diabetic cardiomyopathy. | ||
Drug Deliv Comparative analysis of metabolome and transcriptomes to explore the inhibited influence of sonodynamic therapy combined with lonidamine on hepatocellular carcinoma. | ||
JCI Insight Combination S100A1 and ARC gene therapy as a treatment for DMD cardiomyopathy. | ||
Biochim Biophys Acta Mol Basis Dis Gut-derived 4-hydroxyphenylacetic acid attenuates sepsis-induced acute kidney injury by upregulating ARC to inhibit necroptosis | ||
Cell Cycle Long noncoding RNA PCAT6 inhibits colon cancer cell apoptosis by regulating anti-apoptotic protein ARC expression via EZH2. | ||
J Orthop Surg Res 17β-estradiol promotes osteogenic differentiation of BMSCs by regulating mitophagy through ARC |
