Tested Applications
| Positive WB detected in | PC-3 cells, human pancreas tissue, human skeletal muscle tissue, MCF-7 cells, mouse pancreas tissue, HEK-293 cells, PC-12 cells, NIH/3T3 cells |
| Positive IP detected in | HEK-293T cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells, HCT 116 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11058-1-AP targets NONO in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1512 Product name: Recombinant human NONO protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 124-471 aa of BC010049 Sequence: IAKVELDNMPLRGKQLRVRFACHSASLTVRNLPQYVSNELLEEAFSVFGQVERAVVIVDDRGRPSGKGIVEFSGKPAARKALDRCSEGSFLLTTFPRPVTVEPMDQLDDEEGLPEKLVIKNQQFHKEREQPPRFAQPGSFEYEYAMRWKALIEMEKQQQDQVDRNIKEAREKLEMEMEAARHEHQVMLMRQDLMRRQEELRRMEELHNQEVQKRKQLELRQEEERRRREEEMRRQQEEMMRRQQEGFKGTFPDAREQEIRMGQMAMGGAMGINNRGAMPPAPVPAGTPAPPGPATMMPDGTLGLTPPTTERFGQAATMEGIGAIGGTPPAFNRAAPGAEFAPNKRRRY Predict reactive species |
| Full Name | non-POU domain containing, octamer-binding |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | BC010049 |
| Gene Symbol | NONO |
| Gene ID (NCBI) | 4841 |
| RRID | AB_2152167 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15233 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Non-pou domain-containing octamer-binding protein(NONO), other named p54nrb, belongs to DBHS family, whose protein are predominately nuclear and involves in transcriptional and posttranscriptional gene regulatory functions as well as DNA repair. This family has roles in a wide range of biological processes, such as the regulation of circadian rhythm, carcinogenesis and progression of cancer. NONO forms heterodimer with SFPQ, and has been implicated in nuclear retention of A- to I-edited RNA as paraspeckle components, pre-mRAN 3'-end formation, cAMP cycling and transcriptional activation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NONO antibody 11058-1-AP | Download protocol |
| IHC protocol for NONO antibody 11058-1-AP | Download protocol |
| IP protocol for NONO antibody 11058-1-AP | Download protocol |
| WB protocol for NONO antibody 11058-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-NONO antibody (Cat# 11058-1-AP) has 66 citations across journals including Cell, Nature Metabolism, Nature Genetics, Nature Aging, Nature Cell Biology, Nature Communications, Cancer Research, Molecular Cell, Science Advances, and others. Research fields span paraspeckle/phase separation biology, lncRNA and circRNA regulation, multiple cancer types (breast, liver, gastric, pancreatic, glioblastoma, melanoma), viral infections (SARS-CoV-2, influenza), neurodegeneration (TDP-43, tau, C9orf72), splicing, chromatin biology, and innate immune responses.
| Species | Application | Title |
|---|---|---|
Cell In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs. | ||
Cell Res Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2. | ||
Adv Mater Combating Atherosclerosis with Chirality/Phase Dual-Engineered Nanozyme Featuring Microenvironment-Programmed Senolytic and Senomorphic Actions | ||
Nat Metab Proline exacerbates hepatic gluconeogenesis via paraspeckle-dependent mRNA retention | ||
Nat Genet Computational prediction and experimental validation identify functionally conserved lncRNAs from zebrafish to human | ||
Nat Aging Aging-associated lncRNAs are evolutionarily conserved and participate in NFκB signaling |
Reviews
What customers say
All three reviewers rated this antibody 5 stars. Two used it for Western Blot (at 1/1000 and 1:2000 dilution) in HeLa, Jurkat, and 293T cells, reporting clean bands and calling it "perfect" for detecting NONO. One reviewer used it for immunofluorescence in HeLa cells at 1:1000 dilution with 2 hours incubation at room temperature, noting good nuclear staining. Overall, users report reliable performance across applications with clear, specific results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Roy (Verified Customer) (06-28-2024) | Perfect antibody to detect NONO by WB (Overnight incubation at 4°C, 1/1000 dilution)
|
Xingyuan (Verified Customer) (07-17-2023) | The band quality was decent.
 |
Tatyana (Verified Customer) (05-14-2023) | ICC with antibody diluted in 5% goat serum in PBST, with 2 h incubation at RT. Good nuclear staining
 |
