• Featured Product
  • KD/KO Validated

NRF1/nuclear respiratory factor 1 Antibody

NRF1/nuclear respiratory factor 1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12482-1-AP

Synonyms

ALPHA PAL, NRF 1, NRF1, NRF1/nuclear respiratory factor 1, nuclear respiratory factor 1



Tested Applications

Positive WB detected inA549 cells, human lung tissue, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Sample-dependent, check data in validation data gallery

Product Information

12482-1-AP targets NRF1/nuclear respiratory factor 1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken, goat, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NRF1/nuclear respiratory factor 1 fusion protein Ag3172
Full Name nuclear respiratory factor 1
Calculated molecular weight 522aa,56 kDa
Observed molecular weight 67 kDa, 45 kDa
GenBank accession number BC016925
Gene symbol NRF1
Gene ID (NCBI) 4899
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.

Protocols

Product Specific Protocols
WB protocol for NRF1/nuclear respiratory factor 1 antibody 12482-1-APDownload protocol
IP protocol for NRF1/nuclear respiratory factor 1 antibody 12482-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Mol Nutr Food Res

Sulforaphane Improves Lipid Metabolism by Enhancing Mitochondrial Function and Biogenesis in vivo and vitro.

Authors - Peng Lei
chickenWB

J Agric Food Chem

(-)-Hydroxycitric Acid Suppresses Lipid Droplet Accumulation and Accelerates Energy Metabolism via Activation of the Adiponectin-AMPK Signaling Pathway in Broiler Chickens.

Authors - Longlong Li

Mol Med Rep

PMID: 27779689

Authors - Wei Gao
goatWB

Theriogenology

Effects of nitric oxide on steroidogenesis and apoptosis in goat luteinized granulosa cells.

Authors - Yi-Xuan Guo
ratWB

Evid Based Complement Alternat Med

Modified Si-Ni-San Decoction Ameliorates Central Fatigue by Improving Mitochondrial Biogenesis in the Rat Hippocampus.

Authors - Chenxia Han
mouseWB

PPAR Res

Neuroprotective Effect and Mechanism of Thiazolidinedione on Dopaminergic Neurons In Vivo and In Vitro in Parkinson's Disease.

Authors - Yanqin Wang