NRG1 Antibody

NRG1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10527-1-AP

Synonyms

ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, NRG1, Pro NRG1, SMDF



Tested Applications

Positive WB detected inhuman liver tissue, mouse liver tissue, SH-SY5Y cells
Positive IP detected inmouse liver tissue
Positive IF detected inMCF-7 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10527-1-AP targets NRG1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NRG1 fusion protein Ag0803
Full Name neuregulin 1
Calculated molecular weight 32 kDa
Observed molecular weight 55 kDa and 72 kDa
GenBank accession number BC007675
Gene symbol NRG1
Gene ID (NCBI) 3084
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain.

Protocols

Product Specific Protocols
WB protocol for NRG1 antibody 10527-1-APDownload protocol
IF protocol for NRG1 antibody 10527-1-APDownload protocol
IP protocol for NRG1 antibody 10527-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Am J Transl Res

Therapeutic effects of Erbin inhibitor on spinal cord contusion in mice.

Authors - Yun Wu
mouseWB

Am J Physiol Renal Physiol

ErbB4 deletion accelerates renal fibrosis following renal injury.

Authors - Fenghua Zeng
humanIHC

J Exp Clin Cancer Res

miR-296-5p suppresses EMT of hepatocellular carcinoma via attenuating NRG1/ERBB2/ERBB3 signaling.

Authors - Dong-Min Shi