Tested Applications
| Positive WB detected in | K-562 cells, SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83477-7-RR targets TrkA in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag32759 Product name: Recombinant human NTRK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 537-796 aa of NM_001007792 Sequence: DKMLVAVKALKEASESARQDFQREAELLTMLQHQHIVRFFGVCTEGRPLLMVFEYMRHGDLNRFLRSHGPDAKLLAGGEDVAPGPLGLGQLLAVASQVAAGMVYLAGLHFVHRDLATRNCLVGQGLVVKIGDFGMSRDIYSTDYYRVGGRTMLPIRWMPPESILYRKFTTESDVWSFGVVLWEIFTYGKQPWYQLSNTEAIDCITQGRELERPRACPPEVYAIMRGCWQREPQQRHSIKDVHARLQALAQAPPVYLDVLG Predict reactive species |
| Full Name | neurotrophic tyrosine kinase, receptor, type 1 |
| Calculated Molecular Weight | 88 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | NM_001007792 |
| Gene Symbol | NTRK1 |
| Gene ID (NCBI) | 4914 |
| RRID | AB_3671106 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04629 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NTRK1(Neurotrophic tyrosine kinase receptor 1) is located on chromosome 1q23.1 and is responsible for a rare genetic disorder, CIPA (congenital insensitivity to pain with anhidrosis) (PMID: 37907480). As a neurotrophin receptor, NTRK1 is abundantly expressed in human neuronal tissues and plays an integral role in the development and function of the human nervous system (PMID: 27843590). NTRK1 also regulates the survival and proliferation of cholinergic neurons (PMID: 20054812).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TrkA antibody 83477-7-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review rates the product 5 out of 5 stars. The reviewer used it for immunofluorescence at a 1:100 dilution on human brain cortex tissue. They noted that TRKA (visualized in green) marked some cells in the tissue, though these were not the NeuN-positive neurons (shown in red). Overall, the antibody performed well for its intended application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Reyes (Verified Customer) (10-06-2025) | TRKA (in green) marked some cells of human brain cortex, although not the NeuN-positive neurons (in red)
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