Nestin Monoclonal antibody
Nestin Monoclonal Antibody for WB,ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat
Applications
WB, FC,ELISA
Conjugate
Unconjugated
CloneNo.
1D11H2
Cat no : 66259-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Neuro-2a cells, HEK-293 cells, rat brain tissue, PC-12 cells, rat cerebellum tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 7 publications below |
IHC | See 2 publications below |
IF | See 3 publications below |
FC | See 1 publications below |
Product Information
66259-1-Ig targets Nestin in WB, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | nestin |
Calculated molecular weight | 177 kDa |
Observed molecular weight | 150-170 kDa |
GenBank accession number | NM_006617 |
Gene symbol | NES |
Gene ID (NCBI) | 10763 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Nestin antibody 66259-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancers (Basel) Honokiol Eliminates Glioma/Glioblastoma Stem Cell-Like Cells Via JAK-STAT3 Signaling and Inhibits Tumor Progression by Targeting Epidermal Growth Factor Receptor. | ||
Biomed Res Int In Vitro Neural Differentiation of Bone Marrow Mesenchymal Stem Cells Carrying the FTH1 Reporter Gene and Detection with MRI. | ||
J Allergy Clin Immunol Ras homolog family member A/Rho-associated protein kinase 1 signaling modulates lineage commitment of mesenchymal stem cells in asthmatic patients through lymphoid enhancer-binding factor 1. | ||
Cell Metab Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells. | ||
Cell Death Dis Nonenzymatic function of Aldolase A downregulates miR-145 to promote the Oct4/DUSP4/TRAF4 axis and the acquisition of lung cancer stemness. | ||
EBioMedicine Silencing of circular RNA HIPK2 in neural stem cells enhances functional recovery following ischaemic stroke. |