Nestin Monoclonal antibody

Nestin Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1D11H2

Cat no : 66259-1-Ig

Synonyms

NES, Nestin



Tested Applications

Positive WB detected inNeuro-2a cells, HEK-293 cells, rat brain tissue, PC-12 cells, rat cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

66259-1-Ig targets Nestin in WB, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name nestin
Calculated molecular weight 177 kDa
Observed molecular weight 150-170 kDa
GenBank accession numberNM_006617
Gene symbol NES
Gene ID (NCBI) 10763
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.

Protocols

Product Specific Protocols
WB protocol for Nestin antibody 66259-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancers (Basel)

Honokiol Eliminates Glioma/Glioblastoma Stem Cell-Like Cells Via JAK-STAT3 Signaling and Inhibits Tumor Progression by Targeting Epidermal Growth Factor Receptor.

Authors - Yipu Fan
rat

Biomed Res Int

In Vitro Neural Differentiation of Bone Marrow Mesenchymal Stem Cells Carrying the FTH1 Reporter Gene and Detection with MRI.

Authors - Tong Mu
mouseFC

J Allergy Clin Immunol

Ras homolog family member A/Rho-associated protein kinase 1 signaling modulates lineage commitment of mesenchymal stem cells in asthmatic patients through lymphoid enhancer-binding factor 1.

Authors - Xia Ke
mouseWB

Cell Metab

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Authors - Xiuying Zhong
humanWB

Cell Death Dis

Nonenzymatic function of Aldolase A downregulates miR-145 to promote the Oct4/DUSP4/TRAF4 axis and the acquisition of lung cancer stemness.

Authors - Yu-Chan Chang
mouseWB

EBioMedicine

Silencing of circular RNA HIPK2 in neural stem cells enhances functional recovery following ischaemic stroke.

Authors - Guangtian Wang