Back

Nestin Antibody 2 Publications

Mouse Monoclonal| Catalog number: 66259-1-Ig |CloneNo.: 1D11H2

  • Print page
  • Download PDF

-_-

Freight/Packing

Con: 206 μg/150 μl

Choose size:

submit a review

Please visit your regions distributor:

Request quote for
bulk/custom formulations

Special offer: Add control/tag antibody for $99
Load more
Tested Applications
Applications:
ELISA, IF, IHC, WB
Application not tested? Please see our Guarantee


Images See all

Nestin Antibody WB Neuro-2a cells 66259-1-Ig

Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig( Nestin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Nestin Antibody IHC human gliomas tissue 66259-1-Ig

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using ( Nestin Antibody) at dilution of 1:200 (under 10x lens).

Nestin Antibody IHC human gliomas tissue 66259-1-Ig

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using ( Nestin Antibody) at dilution of 1:200 (under 40x lens).

Nestin Antibody IF U-251 cells 66259-1-Ig

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed U-251 cells using 66259-1-Ig(Nestin antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
Neuro-2a cells, HEK-293 cells, rat brain tissue
Positive IHC detected in:
human gliomas tissue
Positive IF detected in:
U-251 cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:1000
IF : 1:20-1:200

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
nestin
Calculated molecular weight:
177 kDa
Observed molecular weight:
150-170 kDa
GenBank accession number:
NM_006617
Gene ID (NCBI):
10763
Gene symbol
NES
Synonyms
NES, Nestin
Background

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.


Back
to top