Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, mouse heart tissue, Calu-1 cells, mouse lung tissue, rat heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15212-1-AP targets OGDH in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, rabbit, chicken, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7368 Product name: Recombinant human OGDH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 674-1023 aa of BC004964 Sequence: SGQDVERGTFSHRHHVLHDQNVDKRTCIPMNHLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNALVLWEAQFGDFHNTAQCIIDQFICPGQAKWVRQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDVLPDLKEANFDINQLYDCNWVVVNCSTPGNFFHVLRRQILLPFRKPLIIFTPKSLLRHPEARSSFDEMLPGTHFQRVIPEDGPAAQNPENVKRLLFCTGKVYYDLTRERKARDMVGQVAITRIEQLSPFPFDLLLKEVQKYPNAELAWCQEEHKNQGYYDYVKPRLRTTISRAKPVWYAGRDPAAAPATGNKKTHLTELQRLLDTAFDLDVFKNFS Predict reactive species |
| Full Name | oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) |
| Calculated Molecular Weight | 116 kDa |
| Observed Molecular Weight | 116 kDa |
| GenBank Accession Number | BC004964 |
| Gene Symbol | OGDH |
| Gene ID (NCBI) | 4967 |
| RRID | AB_2156759 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q02218 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing. This antibody is immunized with the C-terminal and can recognize the isoform 1 and 2 with the MW of 116 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for OGDH antibody 15212-1-AP | Download protocol |
| IHC protocol for OGDH antibody 15212-1-AP | Download protocol |
| IP protocol for OGDH antibody 15212-1-AP | Download protocol |
| WB protocol for OGDH antibody 15212-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
OGDH Polyclonal antibody (Cat# 15212-1-AP) has accumulated 106 citations across high-impact journals including Nature, Cell Metabolism, Molecular Cell, Nature Communications, Cell Research, Cancer Discovery, and Nature Metabolism. Associated research fields encompass TCA cycle metabolism, mitochondrial function, cancer metabolic reprogramming, neurodegenerative diseases, immunology and inflammation, cardiovascular biology, and hepatology.
| Species | Application | Title |
|---|---|---|
Mol Cancer CBFA2T2: a novel H3K27 reader regulating metabolism and tumor growth.
| ||
J Hepatol OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism. | ||
Cell Metab Dual targeting of SLC25A51 and succinate dehydrogenase selectively depletes mitochondrial NAD(+) to eradicate KRAS-driven AML.
| ||
Cell Metab An Upstream Open Reading Frame in Phosphatase and Tensin Homolog Encodes a Circuit Breaker of Lactate Metabolism. |
