|Positive WB detected in||mouse heart tissue, mouse liver tissue, mouse lung tissue, mouse pancreas tissue, A549 cells, rat heart tissue, rat lung tissue, ROS1728 cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15212-1-AP targets OGDH in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||OGDH fusion protein Ag7368|
|Full Name||oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)|
|Calculated molecular weight||116 kDa|
|Observed molecular weight||116 kDa|
|GenBank accession number||BC004964|
|Gene ID (NCBI)||4967|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing. This antibody is immunized with the C-terminal and can recognize the isoform 1 and 2 with the MW of 116 kDa.
Am J Respir Cell Mol Biol
Glutamine Metabolism is Required for Collagen Protein Synthesis in Lung Fibroblasts.
Regional Molecular Mapping of Primate Synapses during Normal Healthy Aging.
Allopregnanolone Reverses Bioenergetic Deficits in Female Triple Transgenic Alzheimer's Mouse Model.
Two-stage metabolic remodelling in macrophages in response to lipopolysaccharide and interferon-γ stimulation.
Biochem Biophys Res Commun
Ifi27 is indispensable for mitochondrial function and browning in adipocytes.
Potentiation of brain mitochondrial function by S-equol and R/S-equol estrogen receptor β-selective phytoSERM treatments.