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OGDH Monoclonal antibody

OGDH Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1H7A2

Cat no : 66285-1-Ig

Synonyms

AKGDH, E1k, OGDC, OGDC E1, OGDH

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using OGDH Monoclonal antibody (66285-1-Ig)

    WB analysis of human heart using 66285-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 66285-1-Ig (OGDH Antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of ROS1728 cells using OGDH Monoclonal antibody (66285-1-Ig)

    WB analysis of ROS1728 using 66285-1-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66285-1-Ig (OGDH Antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using OGDH Monoclonal antibody (66285-1-Ig)

    IHC staining of human lung cancer using 66285-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66285-1-Ig (OGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using OGDH Monoclonal antibody (66285-1-Ig)

    IHC staining of human lung cancer using 66285-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66285-1-Ig (OGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using OGDH Monoclonal antibody (66285-1-Ig)

    IF Staining of HepG2 using 66285-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using OGDH antibody (66285-1-Ig, Clone: 1H7A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

Tested Applications

Positive WB detected inhuman heart tissue, ROS1728 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66285-1-Ig targets OGDH in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen OGDH fusion protein Ag7484
Full Name oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Calculated Molecular Weight 116 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC004964
Gene Symbol OGDH
Gene ID (NCBI) 4967
RRIDAB_2881668
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for OGDH antibody 66285-1-IgDownload protocol
IHC protocol for OGDH antibody 66285-1-IgDownload protocol
IF protocol for OGDH antibody 66285-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Hum Mol Genet

DHTKD1 and OGDH display substrate overlap in cultured cells and form a hybrid 2-oxo acid dehydrogenase complex in vivo.

Authors - João Leandro
View Article
mouseIHC

PLoS One

Targeting pancreatic cancer with combinatorial treatment of CPI-613 and inhibitors of lactate metabolism.

Authors - Simone Kumstel
View Article