• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

OSTF1 Polyclonal antibody

OSTF1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10671-1-AP

Synonyms

OSF, OSTF1, SH3P2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of C2C12 cells using OSTF1 Polyclonal antibody (10671-1-AP)

    WB analysis of C2C12 using 10671-1-AP

    C2C12 cell were subjected to SDS PAGE followed by western blot with 10671-1-AP (OSTF1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using OSTF1 Polyclonal antibody (10671-1-AP)

    IHC staining of human breast cancer using 10671-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10671-1-AP (OSTF1 antibody) at dilution of 1:100 (under 25x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using OSTF1 Polyclonal antibody (10671-1-AP)

    IHC staining of human kidney using 10671-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using OSTF1 Polyclonal antibody (10671-1-AP)

    IHC staining of human kidney using 10671-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using OSTF1 Polyclonal antibody (10671-1-AP)

    IHC staining of human placenta using 10671-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using OSTF1 Polyclonal antibody (10671-1-AP)

    IHC staining of human placenta using 10671-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using OSTF1 Polyclonal antibody (10671-1-AP)

    IHC staining of human testis using 10671-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using OSTF1 Polyclonal antibody (10671-1-AP)

    IHC staining of human testis using 10671-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using OSTF1 Polyclonal antibody (10671-1-AP)

    IHC staining of human lung using 10671-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using OSTF1 Polyclonal antibody (10671-1-AP)

    IHC staining of human lung using 10671-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 10671-1-AP (OSTF1 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inC2C12 cell
Positive IHC detected inhuman breast cancer tissue, human kidney tissue, human lung tissue, human placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10671-1-AP targets OSTF1 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OSTF1 fusion protein Ag1072
Full Name osteoclast stimulating factor 1
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC007459
Gene Symbol OSTF1
Gene ID (NCBI) 26578
RRIDAB_2156717
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OSTF1 (Osteoclast-stimulating factor-1) is an intracellular protein produced by osteoclasts that indirectly induces osteoclast formation and bone resorption. It induces bone resorption, acting probably through a signaling cascade which results in the secretion of factor(s) enhancing osteoclast formation and activity

Protocols

Product Specific Protocols
WB protocol for OSTF1 antibody 10671-1-APDownload protocol
IHC protocol for OSTF1 antibody 10671-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols