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- KD/KO Validated
P21 Polyclonal antibody
P21 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IP, IHC, IF, FC,ELISA
Conjugate
Unconjugated
295
Cat no : 10355-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, HUVEC cells, HEK-293 cells, HEK-293T cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human cervical cancer tissue, human breast cancer tissue, human colon cancer tissue, human thyroid cancer tissue, human stomach cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells, myotome of a somite at E11.5 tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:200-1:600 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 271 publications below |
| IHC | See 22 publications below |
| IF | See 11 publications below |
| FC | See 1 publications below |
Product Information
10355-1-AP targets P21 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | Artemia, human, swine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | P21 fusion protein Ag0368 |
| Full Name | cyclin-dependent kinase inhibitor 1A (p21, Cip1) |
| Calculated molecular weight | 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC000275 |
| Gene symbol | CDKN1A |
| Gene ID (NCBI) | 1026 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for P21 antibody 10355-1-AP | Download protocol |
| IHC protocol for P21 antibody 10355-1-AP | Download protocol |
| IF protocol for P21 antibody 10355-1-AP | Download protocol |
| IP protocol for P21 antibody 10355-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancers (Basel) Antrocin Sensitizes Prostate Cancer Cells to Radiotherapy through Inhibiting PI3K/AKT and MAPK Signaling Pathways. | ||
EBioMedicine Ubiquinol-cytochrome C reductase core protein II promotes tumorigenesis by facilitating p53 degradation. | ||
Aging (Albany NY) lncRNA-ES3/miR-34c-5p/BMF axis is involved in regulating high-glucose-induced calcification/senescence of VSMCs. | ||
J Cell Physiol Histone demethylase KDM6B regulates 1,25-dihydroxyvitamin D3-induced senescence in glioma cells. | ||
Toxicol In Vitro SIRT1 suppresses p53-dependent apoptosis by modulation of p21 in osteoblast-like MC3T3-E1 cells exposed to fluoride. | ||
Cancer Sci p53/Lactate dehydrogenase A axis negatively regulates aerobic glycolysis and tumor progression in breast cancer expressing wild-type p53. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Susan (Verified Customer) (12-03-2019) | We had been having issues with our old P21 antibody and this one worked great.
|
FH Béatrice (Verified Customer) (09-04-2019) | This is an excellent and specific antibody, without background.When prepared in 3%BSA, it can be reused several times and kept at-20°C
 |
