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P21 Antibody 149 Publications

Rabbit Polyclonal| Catalog number: 10355-1-AP

Featured Product KD/KO validated

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Con: 58 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

P21 Antibody WB HeLa cells 10355-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

P21 Antibody IF MCF-7 cells 10355-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed MCF-7 cells using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

P21 Antibody IF myotome of a somite at E11.5 tissue 10355-1-AP

IF result of anti-p21 (CDKN1A, 10355-1-AP) in the myotome of a somite at E11.5 by Dr. Zalc A. and Dr. Relaix F. (Red = p21; Green=Pax3 from a GFP reporter; Blue=DAPI)

P21 Antibody IHC human cervical cancer tissue 10355-1-AP

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10355-1-AP( P21;CDKN1A Antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

P21 Antibody IHC human cervical cancer tissue 10355-1-AP

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10355-1-AP( P21;CDKN1A Antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

P21 Antibody IP MCF-7 cells 10355-1-AP

IP result of anti-P21;CDKN1A(IP:10355-1-AP, 4ug; Detection:10355-1-AP 1:1000) with MCF-7 cells lysate 2800 ug

P21 Antibody FC HeLa cells 10355-1-AP

1X10^6 HeLa cells were stained with 0.2ug P21;CDKN1A antibody (10355-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, Artemia sinica, swine
Positive WB detected in:
HeLa cells, HUVEC cells, HEK-293 cells, HEK-293T cells, HeLa cells
Positive IP detected in:
MCF-7 cells
Positive IHC detected in:
human cervical cancer tissue, human breast cancer tissue, human colon cancer tissue, human thyroid cancer tissue, human stomach cancer tissue, human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MCF-7 cells, myotome of a somite at E11.5 tissue
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
P21 fusion protein Ag0368
Full name:
cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Calculated molecular weight:
21 kDa
Observed molecular weight:
21 kDa
GenBank accession number:
BC000275
Gene ID (NCBI):
1026
Gene symbol
CDKN1A
Synonyms
CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, p21CIP1, PIC1, SDI1, WAF1

Background

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.


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