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P21 Antibody 172 Publications

Rabbit Polyclonal| Catalog number: 10355-1-AP

Featured Product KD/KO validated

1 customer review

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Con: 58 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

P21 Antibody WB HeLa cells 10355-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10355-1-AP (P21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

P21 Antibody IF MCF-7 cells 10355-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed MCF-7 cells using 10355-1-AP (P21;CDKN1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

P21 Antibody IF myotome of a somite at E11.5 tissue 10355-1-AP

IF result of anti-p21 (CDKN1A, 10355-1-AP) in the myotome of a somite at E11.5 by Dr. Zalc A. and Dr. Relaix F. (Red = p21; Green=Pax3 from a GFP reporter; Blue=DAPI)

P21 Antibody IHC human cervical cancer tissue 10355-1-AP

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10355-1-AP( P21;CDKN1A Antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

P21 Antibody IHC human cervical cancer tissue 10355-1-AP

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10355-1-AP( P21;CDKN1A Antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

P21 Antibody IP MCF-7 cells 10355-1-AP

IP result of anti-P21;CDKN1A(IP:10355-1-AP, 4ug; Detection:10355-1-AP 1:1000) with MCF-7 cells lysate 2800 ug

Species specificity:
human, Artemia sinica, swine
Positive WB detected in:
HeLa cells, HUVEC cells, HEK-293 cells, HEK-293T cells, HeLa cells
Positive IP detected in:
MCF-7 cells
Positive IHC detected in:
human cervical cancer tissue, human breast cancer tissue, human colon cancer tissue, human thyroid cancer tissue, human stomach cancer tissue, human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MCF-7 cells, myotome of a somite at E11.5 tissue
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:200-1:600
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
P21 fusion protein Ag0368
Full name:
cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Calculated molecular weight:
21 kDa
Observed molecular weight:
21 kDa
GenBank accession number:
BC000275
Gene ID (NCBI):
1026
Gene symbol
CDKN1A
Synonyms
CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, p21CIP1, PIC1, SDI1, WAF1

Background

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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P21 Rabbit Polyclonal antibody

Béatrice (Verified Customer), 09-04-2019
Applications
Western Blot,
Primary Antibody Dilution
1/1000
P21 Antibody Western Blot, validation (1/1000 dilution) in HCT116 (Cat no:10355-1-AP)

P21 Antibody Western Blot, validation (1/1000 dilution) in HCT116 (Cat no:10355-1-AP)

Cell/Tissue Type
HCT116
Lot Number
This is an excellent and specific antibody, without background. When prepared in 3%BSA, it can be reused several times and kept at-20°C

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