P21 Antibody 3 Publications

Mouse Monoclonal| Catalog number: 60214-1-Ig |CloneNo.: 5F2F3

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Freight/Packing

Con: 211 μg/150 μl

Choose size:

Please visit your regions distributor:


Species specificity:
human, mouse

Positive WB detected in:
MCF-7 cells,HEK-293 cells, MCF-7 cells

Recommended dilution:
WB : 1:500-1:2000

Product Information


Source:
Mouse

Purification method:
Protein A purification

Isotype:
IgG2b

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
cyclin-dependent kinase inhibitor 1A (p21, Cip1)

Calculated molecular weight:
21 kDa

Observed molecular weight:
21 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
CDKN1A

Synonyms
CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, p21CIP1, PIC1, SDI1, WAF1
Background

CDKN1A (p21, CIP1, WAF1), a cyclin-dependent kinase inhibitor, is necessary for proper control of the cell cycle and premature senescence. P21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. P21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes.


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