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P21 Antibody

P21 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

5F2F3

Cat no : 60214-1-Ig

Synonyms

CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, p21CIP1, PIC1, SDI1, WAF1



Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells, HeLa cells, HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

The 60214-1-Ig immunogen is Fusion Protein P21 protein expressed in E. coli.

Reactivity human
Cited Species human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen P21 fusion protein Ag0308
Full Name cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession number BC000312
Gene symbol CDKN1A
Gene ID (NCBI) 1026
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDKN1A (p21, CIP1, WAF1), a cyclin-dependent kinase inhibitor, is necessary for proper control of the cell cycle and premature senescence. P21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. P21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes.

Protocols

Product Specific Protocols
WB protocol for P21 antibody 60214-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

GTSE1 is involved in breast cancer progression in p53 mutation-dependent manner.

Authors - Fen Lin
humanWB

J Cell Physiol

HOXC13-AS promotes breast cancer cell growth through regulating miR-497-5p/PTEN axis.

Authors - Xiaowei Li
mouseWB

Bosn J Basic Med Sci

CircRNA_014511 affects the radiosensitivity of bone marrow mesenchymal stem cells by binding to miR-29b-2-5p.

Authors - Yanjie Wang
humanWB

Oncol Res Treat

GAS5 Inhibits Gastric Cancer Cell Proliferation Partly by Modulating CDK6.

Authors - Xiaoqiang Guo
humanWB

Biomed Pharmacother

MiRNA-20a-5p promotes the growth of triple-negative breast cancer cells through targeting RUNX3.

Authors - Xiangdong Bai

Mol Med Rep

Ginsenoside Rg1 ameliorates testicular senescence changes in D‑gal‑induced aging mice via anti‑inflammatory and antioxidative mechanisms.

Authors - Zi-Ling Wang