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  • KD/KO Validated

P27; KIP1 Antibody

P27; KIP1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25614-1-AP

Synonyms

CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27, P27; KIP1, P27KIP1



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, NIH/3T3 cells, Jurkat cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue, human colon cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 25614-1-AP immunogen is Fusion Protein P27; KIP1 protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity cow, human, mouse, rat, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P27; KIP1 fusion protein Ag22582
Full Name cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Calculated molecular weight 198aa,22 kDa
Observed molecular weight 27 kDa
GenBank accession number BC001971
Gene symbol CDKN1B
Gene ID (NCBI) 1027
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.

Protocols

Product Specific Protocols
WB protocol for P27; KIP1 antibody 25614-1-APDownload protocol
IHC protocol for P27; KIP1 antibody 25614-1-APDownload protocol
IF protocol for P27; KIP1 antibody 25614-1-APDownload protocol
IP protocol for P27; KIP1 antibody 25614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

J Cell Physiol

Knockdown of TRIM27 expression suppresses the dysfunction of mesangial cells in lupus nephritis by FoxO1 pathway.

Authors - Jinxi Liu
humanWB

J Exp Clin Cancer Res

DRAM2 acts as an oncogene in non-small cell lung cancer and suppresses the expression of p53.

Authors - Muli Wudu
humanWB

Cancer Cell Int

Role of hepatocyte nuclear factor 4 alpha in cell proliferation and gemcitabine resistance in pancreatic adenocarcinoma.

Authors - Qiqing Sun
humanWB

J Agric Food Chem

Avenanthramide A induces cellular senescence via miR-129-3p/Pirh2/p53 signaling pathway to suppress colon cancer growth.

Authors - Rong Fu
ratWB

Stem Cell Res Ther

Autophagy mediates serum starvation-induced quiescence in nucleus pulposus stem cells by the regulation of P27.

Authors - Bin Li
cowWB

Theriogenology

Thyroid hormone (T3) is involved in inhibiting the proliferation of newborn calf Sertoli cells via the PI3K/Akt signaling pathway in vitro.

Authors - Chen Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joshua (Verified Customer) (07-27-2019)

Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Decent staining with some background in rabbit cells. Nuclear localization as expected.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Rabbit Corneal Epithelial Cells
P27; KIP1 Antibody Immunofluorescence, validation (1:500 dilution) in Rabbit Corneal Epithelial Cells (Cat no:25614-1-AP)