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P2RY1 Polyclonal antibody

P2RY1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 18273-1-AP

Synonyms

ATP receptor, P2RY1, P2Y purinoceptor 1, P2Y1, Purinergic receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using P2RY1 Polyclonal antibody (18273-1-AP)

    WB analysis of Jurkat using 18273-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 18273-1-AP (P2RY1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    WB analysis of mouse heart using 18273-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 18273-1-AP (P2RY1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    IHC staining of human testis using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    IHC staining of human testis using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    IHC staining of human kidney using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    IHC staining of human kidney using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    IHC staining of human placenta using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    IHC staining of human placenta using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    IHC staining of human lung using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    IHC staining of human lung using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    IHC staining of human ovary using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using P2RY1 Polyclonal antibody (18273-1-AP)

    IHC staining of human ovary using 18273-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 18273-1-AP (P2RY1 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inJurkat cells, mouse heart tissue
Positive IHC detected inhuman testis tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18273-1-AP targets P2RY1 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P2RY1 fusion protein Ag12992
Full Name purinergic receptor P2Y, G-protein coupled, 1
Calculated Molecular Weight 373 aa, 42 kDa
Observed Molecular Weight 42 kDa, 57 kDa-59 kDa, 66 kDa
GenBank Accession NumberBC074785
Gene Symbol P2RY1
Gene ID (NCBI) 5028
RRIDAB_2158402
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for P2RY1 antibody 18273-1-APDownload protocol
IHC protocol for P2RY1 antibody 18273-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols