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P62/SQSTM1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Cat no : 66184-1-Ig
A170, EBIAP, ORCA, OSIL, p60, P62, P62;SQSTM1, p62B, PDB3, sequestosome 1, SQSTM1, Ubiquitin binding protein p62, ZIP3
Validation Data Gallery
|Positive WB detected in||L02 cells, HEK-293 cells, HepG2 cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Starvation treated HepG2 cells|
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
66184-1-Ig targets P62/SQSTM1 in FC, IF, IHC, WB, ELISA applications and shows reactivity with human samples.
|Cited Species||human, monkey, mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||P62/SQSTM1 fusion protein Ag13131|
|Full Name||sequestosome 1|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||62 kDa|
|GenBank accession number||BC017222|
|Gene ID (NCBI)||8878|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20oC. Aliquoting is unnecessary for -20oC storage.|
Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.
|Product Specific Protocols|
|WB protocol for P62/SQSTM1 antibody 66184-1-Ig||Download protocol|
|IHC protocol for P62/SQSTM1 antibody 66184-1-Ig||Download protocol|
|IF protocol for P62/SQSTM1 antibody 66184-1-Ig||Download protocol|
|FC protocol for P62/SQSTM1 antibody 66184-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
J Cell Physiol
Adiponectin inhibits high glucose-induced angiogenesis via inhibiting autophagy in RF/6A cells.
Acta Biochim Biophys Sin (Shanghai)
Parkin-induced ubiquitination of Mff promotes its association with p62/SQSTM1 during mitochondrial depolarization.
Clinicopathological description of two cases with SQSTM1 gene mutation associated with frontotemporal dementia.
In vivo and in vitro studies using Clonorchis sinensis adult-derived total protein (CsTP) on cellular function and inflammatory effect in mouse and cell model.
Biochem Biophys Res Commun
Rab11a is required for porcine reproductive and respiratory syndrome virus induced autophagy to promote viral replication.
Parkin truncating variants result in a loss-of-function phenotype.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
David Hicks (01-13-2020)
Used for immunocytochemistry. Low signal in control cells, but very strong response when treated with bafilomycin A. Very obvious p62 puncta in cells.