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PABPC3 Polyclonal antibody

PABPC3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12625-2-AP

Synonyms

PABP 3, PABP3, PABPC3, PABPL3, Poly(A) binding protein 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PABPC3 Polyclonal antibody (12625-2-AP)

    WB analysis using 12625-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12625-2-AP (PABPC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PABPC3 Polyclonal antibody (12625-2-AP)

    WB analysis of mouse testis using 12625-2-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12625-2-AP (PABPC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using PABPC3 Polyclonal antibody (12625-2-AP)

    IP experiment of mouse testis using 12625-2-AP

    IP result of anti-PABPC3 (IP:12625-2-AP, 4ug; Detection:12625-2-AP 1:300) with mouse testis tissue lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using PABPC3 Polyclonal antibody (12625-2-AP)

    IHC staining of human testis using 12625-2-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12625-2-AP (PABPC3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PABPC3 Polyclonal antibody (12625-2-AP)

    IHC staining of human testis using 12625-2-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12625-2-AP (PABPC3 antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inrat testis tissue, mouse testis tissue, Jurkat cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12625-2-AP targets PABPC3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PABPC3 fusion protein Ag3317
Full Name poly(A) binding protein, cytoplasmic 3
Calculated Molecular Weight 631 aa, 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC027617
Gene Symbol PABPC3
Gene ID (NCBI) 5042
RRIDAB_2156892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PABPC3 antibody 12625-2-APDownload protocol
IHC protocol for PABPC3 antibody 12625-2-APDownload protocol
IP protocol for PABPC3 antibody 12625-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Assist Reprod Genet

The poly(A)-binding protein genes, EPAB, PABPC1, and PABPC3 are differentially expressed in infertile men with non-obstructive azoospermia.

Authors - Saffet Ozturk
View Article