|Positive WB detected in||NIH/3T3 cells, mouse lung tissue, mouse heart tissue|
|Positive IP detected in||NIH/3T3 cells|
|Positive IHC detected in||human breast cancer tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10518-2-AP targets PACSIN2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PACSIN2 fusion protein Ag0809|
|Full Name||protein kinase C and casein kinase substrate in neurons 2|
|Calculated molecular weight||56 kDa|
|Observed molecular weight||60-65 kDa|
|GenBank accession number||BC008037|
|Gene ID (NCBI)||11252|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Peripheral membrane proteins of the Bin/amphiphysin/Rvs (BAR) and Fer-CIP4 homology-BAR (F-BAR) family participate in cellular membrane trafficking (PMID: 19549836). PACSIN2 (also known as syndapin-2) is a member of the protein kinase C and casein kinase substrate in neurons (PACSIN) family, which are membrane-binding proteins characterized by an amino-terminal F-BAR domain. PACSIN2 plays a role in intracellular vesicle-mediated transport. It is involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization (PMID: 23129763). PACSIN2 also has a role in caveolae membrane sculpting (PMID: 21610094).
|Product Specific Protocols|
|WB protocol for PACSIN2 antibody 10518-2-AP||Download protocol|
|IHC protocol for PACSIN2 antibody 10518-2-AP||Download protocol|
|IF protocol for PACSIN2 antibody 10518-2-AP||Download protocol|
|IP protocol for PACSIN2 antibody 10518-2-AP||Download protocol|
|Click here to view our Standard Protocols|
Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport.
Endogenous Cyclin D1 Promotes the Rate of Onset and Magnitude of Mitogenic Signaling via Akt1 Ser473 Phosphorylation.
Application of a C. trachomatis expression system to identify C. pneumoniae proteins translocated into host cells.
Role of Phosphatidylinositol 4,5-Bisphosphate in Regulating EHD2 Plasma Membrane Localization.
Mol Biol Cell
Cooperation of MICAL-L1, syndapin2, and phosphatidic acid in tubular recycling endosome biogenesis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Herve (Verified Customer) (11-13-2019)
This antibody recognizes a 55-kDa band that is present in both Pacsin2 WT and KO platelet lysates. PACSIN2 runs normally at 65 kDa in human and mouse platelet lysates and is absent in mouse Pacsin2 KO samples.