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  • KD/KO Validated

PACSIN2 Polyclonal antibody

PACSIN2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10518-2-AP

Synonyms

PACSIN2, SDPII, Syndapin 2



Tested Applications

Positive WB detected inNIH/3T3 cells, mouse lung tissue, mouse heart tissue
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10518-2-AP targets PACSIN2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PACSIN2 fusion protein Ag0809
Full Name protein kinase C and casein kinase substrate in neurons 2
Calculated molecular weight 56 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberBC008037
Gene symbol PACSIN2
Gene ID (NCBI) 11252
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Peripheral membrane proteins of the Bin/amphiphysin/Rvs (BAR) and Fer-CIP4 homology-BAR (F-BAR) family participate in cellular membrane trafficking (PMID: 19549836). PACSIN2 (also known as syndapin-2) is a member of the protein kinase C and casein kinase substrate in neurons (PACSIN) family, which are membrane-binding proteins characterized by an amino-terminal F-BAR domain. PACSIN2 plays a role in intracellular vesicle-mediated transport. It is involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization (PMID: 23129763). PACSIN2 also has a role in caveolae membrane sculpting (PMID: 21610094).

Protocols

Product Specific Protocols
WB protocol for PACSIN2 antibody 10518-2-APDownload protocol
IHC protocol for PACSIN2 antibody 10518-2-APDownload protocol
IF protocol for PACSIN2 antibody 10518-2-APDownload protocol
IP protocol for PACSIN2 antibody 10518-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouse,zebrafishWB,IF

Nat Commun

Investigation of F-BAR domain PACSIN proteins uncovers membrane tubulation function in cilia assembly and transport.

Authors - Christine Insinna
  • KO Validated
humanWB

Cell Rep

Endogenous Cyclin D1 Promotes the Rate of Onset and Magnitude of Mitogenic Signaling via Akt1 Ser473 Phosphorylation.

Authors - Ke Chen
humanWB,IF

J Bacteriol

Application of a C. trachomatis expression system to identify C. pneumoniae proteins translocated into host cells.

Authors - Izumi Yanatori
humanWB

PLoS One

Role of Phosphatidylinositol 4,5-Bisphosphate in Regulating EHD2 Plasma Membrane Localization.

Authors - Simone Laura C LC
  • KD Validated
humanWB,IF

Mol Biol Cell

Cooperation of MICAL-L1, syndapin2, and phosphatidic acid in tubular recycling endosome biogenesis.

Authors - Giridharan Sai Srinivas Panapakkam SS
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Herve (Verified Customer) (11-13-2019)

This antibody recognizes a 55-kDa band that is present in both Pacsin2 WT and KO platelet lysates. PACSIN2 runs normally at 65 kDa in human and mouse platelet lysates and is absent in mouse Pacsin2 KO samples.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Platelets