PAI-1 Polyclonal antibody

PAI-1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 25522-1-AP

Synonyms

PAI, PAI 1, PAI1, PLANH1, Serpin E1, SERPINE1



Tested Applications

Positive WB detected inRAW 264.7 cells, human plasma tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25522-1-AP is PAI-1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAI-1 fusion protein Ag22411
Full Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Calculated molecular weight 402 aa, 45 kDa
GenBank accession numberBC010860
Gene symbol SERPINE1
Gene ID (NCBI) 5054
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tissue plasminogen activator (tPA) and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia.

Protocols

Product Specific Protocols
WB protocol for PAI-1 antibody 25522-1-APDownload protocol
IF protocol for PAI-1 antibody 25522-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols