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PAI-1 Polyclonal antibody

PAI-1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25522-1-AP

Synonyms

PAI, PAI 1, PAI1, PLANH1, Serpin E1, SERPINE1

Validation Data Gallery

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  • Western Blot (WB) analysis of RAW 264.7 cells using PAI-1 Polyclonal antibody (25522-1-AP)

    WB analysis of RAW 264.7 using 25522-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 25522-1-AP (PAI-1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using PAI-1 Polyclonal antibody (25522-1-AP)

    WB analysis of human plasma using 25522-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 25522-1-AP (PAI-1 antibody at dilution of 1:300 incubated at 4 degree celsius over night.

Tested Applications

Positive WB detected inRAW 264.7 cells, human plasma tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 25522-1-AP is PAI-1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAI-1 fusion protein Ag22411
Full Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Calculated Molecular Weight 402 aa, 45 kDa
GenBank Accession NumberBC010860
Gene Symbol SERPINE1
Gene ID (NCBI) 5054
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tissue plasminogen activator (tPA) and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia.

Protocols

Product Specific Protocols
WB protocol for PAI-1 antibody 25522-1-APDownload protocol
IF protocol for PAI-1 antibody 25522-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Cell Dev Biol

The predictive effect of immune therapy and chemotherapy under T cell-related gene prognostic index for Gastric cancer

Authors - Jingyao Chen
View Article