|Positive WB detected in||NIH/3T3 cells, HepG2 cells|
|Positive IHC detected in||human heart tissue, human brain tissue, human kidney tissue, human liver tissue, human placenta tissue, human spleen tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16427-1-AP targets PAN2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PAN2 fusion protein Ag9670|
|Full Name||PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae)|
|Calculated molecular weight||1198aa,135 kDa|
|Observed molecular weight||127 kDa|
|GenBank accession number||BC024043|
|Gene ID (NCBI)||9924|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN).
Anti-PABPC1 Co-Immunoprecipitation for Examining the miRNAs Directly Targeting the 3'-UTR of EED mRNA.
Mobilization of Dormant Cnot7 mRNA Promotes Deadenylation of Maternal Transcripts During Mouse Oocyte Maturation.
Nat Struct Mol Biol
An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to mediate mRNA deadenylation and decay.
USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization.