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  • KD/KO Validated

PAN2 Antibody

PAN2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16427-1-AP

Synonyms

hPAN2, KIAA0710, PAN2, USP52



Tested Applications

Positive WB detected inNIH/3T3 cells, HepG2 cells
Positive IHC detected inhuman heart tissue, human brain tissue, human kidney tissue, human liver tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16427-1-AP targets PAN2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAN2 fusion protein Ag9670
Full Name PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae)
Calculated molecular weight 1198aa,135 kDa
Observed molecular weight 127 kDa
GenBank accession number BC024043
Gene symbol PAN2
Gene ID (NCBI) 9924
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PAN2 antibody 16427-1-APDownload protocol
IHC protocol for PAN2 antibody 16427-1-APDownload protocol
IF protocol for PAN2 antibody 16427-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cells

Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN).

Authors - Tian-Li Duan
humanWB, IP

PLoS One

Anti-PABPC1 Co-Immunoprecipitation for Examining the miRNAs Directly Targeting the 3'-UTR of EED mRNA.

Authors - Yi Hu
mouseWB

Biol Reprod

Mobilization of Dormant Cnot7 mRNA Promotes Deadenylation of Maternal Transcripts During Mouse Oocyte Maturation.

Authors - Jun Ma
WB

Nat Struct Mol Biol

An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to mediate mRNA deadenylation and decay.

Authors - Stefanie Jonas
humanWB,IHC,IF

Nat Commun

USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization.

Authors - Shangda Yang
  • KD Validated