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  • Featured Product
  • KD/KO Validated

PANK1 Polyclonal antibody

PANK1 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11768-1-AP

Synonyms

hPanK, hPanK1, PANK, PANK1, PANK1a, PANK1b, pantothenate kinase 1, Pantothenic acid kinase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using PANK1 Polyclonal antibody (11768-1-AP)

    WB analysis of Raji using 11768-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 11768-1-AP (PANK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using PANK1 Polyclonal antibody (11768-1-AP)

    WB analysis of human heart using 11768-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11768-1-AP (PANK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SW 1990 cells using PANK1 Polyclonal antibody (11768-1-AP)

    WB analysis of SW 1990 using 11768-1-AP

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 11768-1-AP (PANK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PANK1 Polyclonal antibody (11768-1-AP)

    WB analysis of MCF-7 using 11768-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11768-1-AP (PANK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PANK1 Polyclonal antibody (11768-1-AP)

    WB analysis using 11768-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11768-1-AP (PANK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inRaji cells, SW 1990 cells, HeLa cells, human heart tissue, MCF-7 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11768-1-AP targets PANK1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PANK1 fusion protein Ag2324
Full Name pantothenate kinase 1
Calculated Molecular Weight 598 aa, 64 kDa
Observed Molecular Weight 61-64 kDa, 40-45 kDa
GenBank Accession NumberBC026296
Gene Symbol PANK1
Gene ID (NCBI) 53354
RRIDAB_2158913
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PANK1(Pantothenate kinase 1) is also named as PANk and belongs to the type II pantothenate kinase family. The PANK1 protein, which catalyzes the rate-limiting step of coenzyme A biosynthesis, is also upregulated by p53(PMID:20833636). It also catalyzes the cytosolic phosphorylation of pantothenate (vitamin B5) and derivatives. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 64 kDa, 42 kDa, 36kDa and 45 kDa,respectively.

Protocols

Product Specific Protocols
WB protocol for PANK1 antibody 11768-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cell Mol Med

PMID: 32048816

Authors - Lu Yang
  • KD Validated
View Article
humanWB

Int J Biol Sci

Pantothenate Kinase 1 Inhibits the Progression of Hepatocellular Carcinoma by Negatively Regulating Wnt/β-catenin Signaling.

Authors - Yuyuan Zi
  • KD Validated
View Article
humanWB

Mol Med Rep

Dioscin inhibits SCC15 cell proliferation via the RASSF1A/MST2/YAP axis.

Authors - Hui Tian
View Article