PARG Polyclonal antibody

PARG Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27808-1-AP

Synonyms

PARG, PARG99



Tested Applications

Positive WB detected inK-562 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27808-1-AP targets PARG in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivityhuman, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PARG fusion protein Ag27137
Full Name poly (ADP-ribose) glycohydrolase
Calculated Molecular Weight 111 kDa
Observed Molecular Weight 100-111 kDa
GenBank Accession NumberBC052966
Gene Symbol PARG
Gene ID (NCBI) 8505
RRIDAB_2880979
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PARG antibody 27808-1-APDownload protocol
IHC protocol for PARG antibody 27808-1-APDownload protocol
IP protocol for PARG antibody 27808-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Theranostics

Hepatoma cell-intrinsic TLR9 activation induces immune escape through PD-L1 upregulation in hepatocellular carcinoma.

Authors - Binghai Zhou
zebrafishWB

Int J Mol Sci

Synthesis and Degradation of Poly(ADP-ribose) in Zebrafish Brain Exposed to Aluminum

Authors - Anna Rita Bianchi
humanWB,IF

Viruses

Role of PARP-1 in Human Cytomegalovirus Infection and Functional Partners Encoded by This Virus

Authors - Wenchang Zhang