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PC Antibody

PC Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4F5D1

Cat no : 66615-1-Ig

Synonyms

PC, PCB, pyruvate carboxylase, Pyruvic carboxylase



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66615-1-Ig targets PC in WB, IHC, IF, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PC fusion protein Ag8846
Full Name pyruvate carboxylase
Calculated molecular weight 1178aa,130 kDa
Observed molecular weight 130 kDa
GenBank accession number BC011617
Gene symbol PC
Gene ID (NCBI) 5091
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PC antibody 66615-1-IgDownload protocol
IHC protocol for PC antibody 66615-1-IgDownload protocol
IF protocol for PC antibody 66615-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols