Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, HSC-T6 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66615-1-Ig targets Pyruvate Carboxylase in WB, IHC, RIP, ELISA applications and shows reactivity with Human, Rat , mouse samples.
| Tested Reactivity | Human, Rat , mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8846 Product name: Recombinant human PC protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 828-1178 aa of BC011617 Sequence: MERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE Predict reactive species |
| Full Name | pyruvate carboxylase |
| Calculated Molecular Weight | 1178 aa, 130 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC011617 |
| Gene Symbol | Pyruvate Carboxylase |
| Gene ID (NCBI) | 5091 |
| RRID | AB_2881975 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P11498 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Pyruvate Carboxylase antibody 66615-1-Ig | Download protocol |
| WB protocol for Pyruvate Carboxylase antibody 66615-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Pyruvate Carboxylase Monoclonal antibody (4F5D1), catalog number 66615-1-Ig, has 5 total citations. These appear in Cell Metabolism, Journal of Nutritional Biochemistry, Frontiers in Oncology, bioRxiv, and eLife. The research fields span metabolic fitness and cognitive performance during sleep disruption, pyruvate carboxylase targeting for acute gastric ulcer treatment, lung adenocarcinoma progression and chemotherapy sensitivity, aspartate metabolism in succinate dehydrogenase-deficient cancer cells, and mitochondrial redox adaptations in alternative aspartate synthesis.
| Species | Application | Title |
|---|---|---|
Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption | ||
J Nutr Biochem Discovery of myristic acid, from chick early amniotic fluid for the treatment of acute gastric ulcers by targeting pyruvate carboxylase to improve TCA cycle. | ||
Front Oncol piR-hsa-211106 Inhibits the Progression of Lung Adenocarcinoma Through Pyruvate Carboxylase and Enhances Chemotherapy Sensitivity. | ||
bioRxiv Adaptive plasticity of aspartate metabolism in succinate dehydrogenase-deficient cancer cells.
| ||
Elife Mitochondrial redox adaptations enable alternative aspartate synthesis in SDH-deficient cells
|
