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  • Western Blot (WB) analysis of human skeletal muscle tissue using PCBD2 Polyclonal antibody (11958-1-AP)

    WB analysis of human skeletal muscle using 11958-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11958-1-AP (PCBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using PCBD2 Polyclonal antibody (11958-1-AP)

    WB analysis of human skeletal muscle using 11958-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11958-1-AP (PCBD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PCBD2 Polyclonal antibody

PCBD2 Polyclonal Antibody for WB, ELISA
Cat No. 11958-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DcoH like protein DCoHm, DCOH2, DCOHM, PCBD2, PHS 2, PHS2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inhuman skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11958-1-AP targets PCBD2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2562

Product name: Recombinant human PCBD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-130 aa of BC054021

Sequence: MAAVLGALGATRRLLAALRGQSLGLAAMSSGTHRLTAEERNQAILDLKAAGWSELSERDAIYKEFSFHNFNQAFGFMSRVALQAEKMNHHPEWFNVYNKVQITLTSHDCGELTKKDVKLAKFIEKAAASV

Predict reactive species
Full Name pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) 2
Calculated Molecular Weight 130 aa, 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC054021
Gene Symbol PCBD2
Gene ID (NCBI) 84105
RRIDAB_2877806
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0N5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCBD2(Pterin-4-alpha-carbinolamine dehydratase 2) is also named DCOH2, DCOHM and belongs to the pterin-4-alpha-carbinolamine dehydratase family. It is involved in tetrahydrobiopterin biosynthesis and it seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. PCBD2 also regulates the dimerization of homeodomain protein HNF-1-alpha and enhances its transcriptional activity.

Protocols

Product Specific Protocols
WB protocol for PCBD2 antibody 11958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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