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PCNP Polyclonal antibody

PCNP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11180-2-AP

Synonyms

PCNP



Tested Applications

Positive WB detected inJurkat cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11180-2-AP targets PCNP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCNP fusion protein Ag1663
Full Name PEST proteolytic signal containing nuclear protein
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 24-28 kDa
GenBank Accession NumberBC013916
Gene Symbol PCNP
Gene ID (NCBI) 57092
RRIDAB_2160652
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCNP can be detected as 24-28 kDa after posttranslational modification.

Protocols

Product Specific Protocols
WB protocol for PCNP antibody 11180-2-APDownload protocol
IHC protocol for PCNP antibody 11180-2-APDownload protocol
IF protocol for PCNP antibody 11180-2-APDownload protocol
IP protocol for PCNP antibody 11180-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Mol Med

PCNP promotes ovarian cancer progression by accelerating β-catenin nuclear accumulation and triggering EMT transition.

Authors - Pengzhen Dong
humanWB,IHC,IF

BMC Cancer

PEST-containing nuclear protein mediates the proliferation, migration, and invasion of human neuroblastoma cells through MAPK and PI3K/AKT/mTOR signaling pathways.

Authors - Dong-Dong Wu
  • KD Validated