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PCYT1B Polyclonal antibody

PCYT1B Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 13765-1-AP

Synonyms

CCT B, CCT beta, CCTB, CT B, CTB, PCYT1B

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using PCYT1B Polyclonal antibody (13765-1-AP)

    WB analysis of human placenta using 13765-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 13765-1-AP (PCYT1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PCYT1B Polyclonal antibody (13765-1-AP)

    WB analysis of human brain using 13765-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13765-1-AP (PCYT1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of human placenta tissue using PCYT1B Polyclonal antibody (13765-1-AP)

    IP experiment of human placenta using 13765-1-AP

    IP result of anti-PCYT1B (IP:13765-1-AP, 4ug; Detection:13765-1-AP 1:500) with human placenta tissue lysate 4000ug.

Tested Applications

Positive WB detected inhuman placenta tissue, human brain tissue
Positive IP detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13765-1-AP targets PCYT1B in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCYT1B fusion protein Ag4748
Full Name phosphate cytidylyltransferase 1, choline, beta
Calculated Molecular Weight 351 aa, 40 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC045634
Gene Symbol PCYT1B
Gene ID (NCBI) 9468
RRIDAB_10642953
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PCYT1B antibody 13765-1-APDownload protocol
IP protocol for PCYT1B antibody 13765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neurobiol Dis

Choline metabolism in regulating inflammatory bowel disease-linked anxiety disorders: A multi-omics exploration of the gut-brain axis

Authors - Fan Zhang
View Article