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  • KD/KO Validated

CCM3/PDCD10 Polyclonal antibody

CCM3/PDCD10 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10294-2-AP

Synonyms

CCM3, PDCD10, programmed cell death 10, TFAR15



Tested Applications

Positive WB detected inK-562 cells, HeLa cells, rat skin tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:100-1:500
Sample-dependent, check data in validation data gallery

Product Information

10294-2-AP targets CCM3/PDCD10 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCM3/PDCD10 fusion protein Ag0348
Full Name programmed cell death 10
Calculated molecular weight 25 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC002506
Gene symbol PDCD10
Gene ID (NCBI) 11235
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDCD10, also named CCM3 and TFAR15, belongs to the PDCD10 family. PDCD10 promotes cell proliferation, increases MAPK activity and modulates apoptotic pathways. PDCD10 is important for cell migration and for normal structure and assembly of the Golgi complex. PDCD10 is required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development, moreover, defects in PDCD10 showed abnormal cardiovascular development. Catalog#10294-2-AP is a rabbit polyclonal antibody raised against the full-length PDCD10 of human origin.

Protocols

Product Specific Protocols
WB protocol for CCM3/PDCD10 antibody 10294-2-APDownload protocol
IHC protocol for CCM3/PDCD10 antibody 10294-2-APDownload protocol
IP protocol for CCM3/PDCD10 antibody 10294-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,ratWB,IF

Brain Res Bull

Cerebral cavernous malformation 3 relieves subarachnoid hemorrhage-induced neuroinflammation in rats through inhibiting NF-kB signaling pathway.

Authors - Wei Peng

Toxicol Lett

Arsenic-induced anti-angiogenesis via miR-425-5p-regulated CCM3.

Authors - Yanfang Gao
humanWB,IHC

J Biol Chem

PDCD10/CCM3 acts downstream of {gamma}-protocadherins to regulate neuronal survival.

Authors - Lin Chengyi C
  • KD Validated
humanWB

J Biol Chem

Adaptor protein cerebral cavernous malformation 3 (CCM3) mediates phosphorylation of the cytoskeletal proteins ezrin/radixin/moesin by mammalian Ste20-4 to protect cells from oxidative stress.

Authors - Fidalgo Miguel M
  • KD Validated
ratWB

Biochem J

A novel non-canonical mechanism of regulation of MST3 (mammalian Sterile20-related kinase 3).

Authors - Fuller Stephen J SJ
humanWB

Hum Mutat

A single-center study on 140 patients with cerebral cavernous malformations: 28 new pathogenic variants and functional characterization of a PDCD10 large deletion ​.

Authors - Grazia Nardella