PDK1 Polyclonal antibody

PDK1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18262-1-AP

Synonyms

PDH kinase 1, PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1



Tested Applications

Positive WB detected inmouse liver tissue, HEK-293 cells, NIH/3T3 cells, rat kidney tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

18262-1-AP targets PDK1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDK1 fusion protein Ag13121
Full Name pyruvate dehydrogenase kinase, isozyme 1
Calculated molecular weight 436 aa, 49 kDa
Observed molecular weight 46-55 kDa
GenBank accession numberBC039158
Gene symbol PDK1
Gene ID (NCBI) 5163
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDK1(pyruvate dehydrogenase kinase isoform 1) is also named as PDHK1 and belongs to the PDK/BCKDK protein kinase family. It inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism (PMID:17683942). Starvation increases islet PDK activity (PMID:11723055). PDK1 has 2 isoforms with the MW of 49 and 52 kD, and a 28aa-transit-peptide in the N-terminal can be removed in the mature form. This antibody is specific to PDK1.

Protocols

Product Specific Protocols
WB protocol for PDK1 antibody 18262-1-APDownload protocol
IHC protocol for PDK1 antibody 18262-1-APDownload protocol
IF protocol for PDK1 antibody 18262-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

The SIAH2-NRF1 axis spatially regulates tumor microenvironment remodeling for tumor progression.

Authors - Biao Ma
ratWB

Artif Organs

Donor Treatment With a Hypoxia-Inducible Factor-1 Agonist Prevents Donation After Cardiac Death Liver Graft Injury in a Rat Isolated Perfusion Model.

Authors - Xingjian Zhang
humanWB

Redox Biol

Impairment of Akt activity by CYP2E1 mediated oxidative stress is involved in chronic ethanol-induced fatty liver.

Authors - Tao Zeng