PER3 Polyclonal antibody

PER3 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12550-1-AP

Synonyms

GIG13, hPER3, PER3, period homolog 3 (Drosophila)



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

12550-1-AP targets PER3 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PER3 fusion protein Ag3400
Full Name period homolog 3 (Drosophila)
Calculated molecular weight 1201 aa, 132 kDa
Observed molecular weight 132 kDa
GenBank accession numberBC026102
Gene symbol PER3
Gene ID (NCBI) 8863
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The suprachiasmatic nuclei (SCN) of the anterior hypothalamus function as a circadian clock in the mammalian brain, generating circadian rhythms in physiology and behavior. The period homolog genesPer3 is an important component of the circadian clock system. PER3 plays a role in cell proliferation, apoptosis,DNA damage response, cell cycle control, and treatment response of chemotherapy agents in cancers.

Protocols

Product Specific Protocols
WB protocol for PER3 antibody 12550-1-APDownload protocol
IHC protocol for PER3 antibody 12550-1-APDownload protocol
IF protocol for PER3 antibody 12550-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

Loss of circadian protein TIMELESS accelerates the progression of cellular senescence.

Authors - Xiaomeng Shen
humanWB

Int J Biochem Cell Biol

TFEB Regulates PER3 Expression via Glucose-dependent Effects on CLOCK/BMAL1.

Authors - Wenwen Luo