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PER3 Polyclonal antibody

PER3 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12550-1-AP

Synonyms

GIG13, hPER3, PER3, period homolog 3 (Drosophila)

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PER3 Polyclonal antibody (12550-1-AP)

    WB analysis of HeLa using 12550-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12550-1-AP (PER3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PER3 Polyclonal antibody (12550-1-AP)

    IHC staining of human colon cancer using 12550-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12550-1-AP (PER3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PER3 Polyclonal antibody (12550-1-AP)

    IF Staining of HeLa using 12550-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12550-1-AP (PER3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

12550-1-AP targets PER3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PER3 fusion protein Ag3400
Full Name period homolog 3 (Drosophila)
Calculated Molecular Weight 1201 aa, 132 kDa
Observed Molecular Weight 132 kDa
GenBank Accession NumberBC026102
Gene Symbol PER3
Gene ID (NCBI) 8863
RRIDAB_2161814
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The suprachiasmatic nuclei (SCN) of the anterior hypothalamus function as a circadian clock in the mammalian brain, generating circadian rhythms in physiology and behavior. The period homolog genesPer3 is an important component of the circadian clock system. PER3 plays a role in cell proliferation, apoptosis,DNA damage response, cell cycle control, and treatment response of chemotherapy agents in cancers.

Protocols

Product Specific Protocols
WB protocol for PER3 antibody 12550-1-APDownload protocol
IHC protocol for PER3 antibody 12550-1-APDownload protocol
IF protocol for PER3 antibody 12550-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Arthritis Res Ther

Dark-light cycle disrupts bone metabolism and suppresses joint deterioration in osteoarthritic rats.

Authors - Xiaopeng Song
View Article
humanWB

Int J Biochem Cell Biol

TFEB Regulates PER3 Expression via Glucose-dependent Effects on CLOCK/BMAL1.

Authors - Wenwen Luo
View Article
humanWB

Biochem Biophys Res Commun

Loss of circadian protein TIMELESS accelerates the progression of cellular senescence.

Authors - Xiaomeng Shen
View Article
humanWB

Adv Sci (Weinh)

The PRMT6/PARP1/CRL4B Complex Regulates the Circadian Clock and Promotes Breast Tumorigenesis

Authors - Tianshu Yang
View Article
mouseWB

Anesth Analg

Sleep Fragmentation, Electroencephalographic Slowing, and Circadian Disarray in a Mouse Model for Intensive Care Unit Delirium

Authors - Elzbieta Dulko
View Article