PFKFB1 Polyclonal antibody

PFKFB1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21718-1-AP

Synonyms

6PF 2 K/Fru 2,6 P2ase 1, F6PK, HL2K, PFK/FBPase 1, PFKFB1, PFRX



Tested Applications

Positive WB detected inHepG2 cells, SMM-7721 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21718-1-AP is PFKFB1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PFKFB1 fusion protein Ag16362
Full Name 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
Calculated molecular weight 471 aa, 55 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC096079
Gene symbol PFKFB1
Gene ID (NCBI) 5207
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PFKFB1 antibody 21718-1-APDownload protocol
IHC protocol for PFKFB1 antibody 21718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols