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- KD/KO Validated
PGM1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 15161-1-AP
Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1
Validation Data Gallery
|Positive WB detected in||mouse heart tissue, HEK-293 cells, HeLa cells, Jurkat cells, mouse skin tissue|
|Positive IP detected in||mouse skin tissue|
|Positive IHC detected in||human testis tissue, human hepatocirrhosis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
15161-1-AP targets PGM1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PGM1 fusion protein Ag7269|
|Full Name||phosphoglucomutase 1|
|Calculated molecular weight||61 kDa|
|Observed molecular weight||61 kDa|
|GenBank accession number||BC001756|
|Gene ID (NCBI)||5236|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.
|Product Specific Protocols|
|WB protocol for PGM1 antibody 15161-1-AP||Download protocol|
|IHC protocol for PGM1 antibody 15161-1-AP||Download protocol|
|IF protocol for PGM1 antibody 15161-1-AP||Download protocol|
|IP protocol for PGM1 antibody 15161-1-AP||Download protocol|
|FC protocol for PGM1 antibody 15161-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Inherit Metab Dis
A novel congenital disorder of glycosylation type without central nervous system involvement caused by mutations in the phosphoglucomutase 1 gene.
C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.
Fibroblasts Mobilize Tumor Cell Glycogen to Promote Proliferation and Metastasis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Patricia (Verified Customer) (02-25-2020)
Works very well.