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PGM1 Antibody

PGM1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15161-1-AP

Synonyms

Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1



Tested Applications

Positive WB detected inmouse heart tissue, HEK-293 cells, HeLa cells, Jurkat cells, mouse skin tissue
Positive IP detected inmouse skin tissue
Positive IHC detected inhuman testis tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15161-1-AP targets PGM1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PGM1 fusion protein Ag7269
Full Name phosphoglucomutase 1
Calculated molecular weight 61 kDa
Observed molecular weight 61 kDa
GenBank accession number BC001756
Gene symbol PGM1
Gene ID (NCBI) 5236
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PGM1 antibody 15161-1-APDownload protocol
IHC protocol for PGM1 antibody 15161-1-APDownload protocol
IF protocol for PGM1 antibody 15161-1-APDownload protocol
IP protocol for PGM1 antibody 15161-1-APDownload protocol
FC protocol for PGM1 antibody 15161-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Inherit Metab Dis

A novel congenital disorder of glycosylation type without central nervous system involvement caused by mutations in the phosphoglucomutase 1 gene.

Authors - Pérez Belén B
humanWB

Brain

C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.

Authors - Scott P Allen
humanWB,IF

Cell Metab

Fibroblasts Mobilize Tumor Cell Glycogen to Promote Proliferation and Metastasis.

Authors - Marion Curtis
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Patricia (Verified Customer) (02-25-2020)

Works very well.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Ovarian cancer
No image submitted with review