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PHKA1 Polyclonal antibody

PHKA1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24279-1-AP

Synonyms

PHKA, PHKA1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse skeletal muscle tissue using PHKA1 Polyclonal antibody (24279-1-AP)

    WB analysis of mouse skeletal muscle using 24279-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24279-1-AP (PHKA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using PHKA1 Polyclonal antibody (24279-1-AP)

    WB analysis of mouse heart using 24279-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 24279-1-AP (PHKA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PHKA1 Polyclonal antibody (24279-1-AP)

    IHC staining of human skeletal muscle using 24279-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24279-1-AP (PHKA1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PHKA1 Polyclonal antibody (24279-1-AP)

    IHC staining of human skeletal muscle using 24279-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24279-1-AP (PHKA1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PHKA1 Polyclonal antibody (24279-1-AP)

    IF Staining of HeLa using 24279-1-AP

    Immunofluorescent analysis of HeLa cells using 24279-1-AP (PHKA1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, mouse heart tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24279-1-AP is PHKA1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHKA1 fusion protein Ag17998
Full Name phosphorylase kinase, alpha 1 (muscle)
Calculated Molecular Weight 1223 aa, 137 kDa
Observed Molecular Weight 130 / 140 kDa
GenBank Accession NumberBC104944
Gene Symbol PHKA1
Gene ID (NCBI) 5255
RRIDAB_2879467
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PHKA1 antibody 24279-1-APDownload protocol
IHC protocol for PHKA1 antibody 24279-1-APDownload protocol
IF protocol for PHKA1 antibody 24279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Transl Med

Identification of molecular subtypes and a novel prognostic model of diffuse large B-cell lymphoma based on a metabolism-associated gene signature.

Authors - Jing He
View Article
mouseWB

Sci Adv

Lactate metabolism is essential in early-onset mitochondrial myopathy

Authors - Zhenkang Chen
View Article