PHKA1 Polyclonal antibody

PHKA1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24279-1-AP

Synonyms

PHKA, PHKA1



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, mouse heart tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24279-1-AP is PHKA1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHKA1 fusion protein Ag17998
Full Name phosphorylase kinase, alpha 1 (muscle)
Calculated molecular weight 1223 aa, 137 kDa
Observed molecular weight 130 / 140 kDa
GenBank accession numberBC104944
Gene symbol PHKA1
Gene ID (NCBI) 5255
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PHKA1 antibody 24279-1-APDownload protocol
IHC protocol for PHKA1 antibody 24279-1-APDownload protocol
IF protocol for PHKA1 antibody 24279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols