PHKG2 Polyclonal antibody

PHKG2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15109-1-AP

Synonyms

GSD9C, PHK gamma T, PHKG2, PHK-gamma-LT, Phosphorylase kinase subunit gamma-2, PSK C3



Tested Applications

Positive WB detected inHEK-293 cells, mouse testis tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15109-1-AP targets PHKG2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHKG2 fusion protein Ag6829
Full Name phosphorylase kinase, gamma 2 (testis)
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC002541
Gene Symbol PHKG2
Gene ID (NCBI) 5261
RRIDAB_2165529
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PHKG2 antibody 15109-1-APDownload protocol
IHC protocol for PHKG2 antibody 15109-1-APDownload protocol
IF protocol for PHKG2 antibody 15109-1-APDownload protocol
IP protocol for PHKG2 antibody 15109-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Adv

Lactate metabolism is essential in early-onset mitochondrial myopathy

Authors - Zhenkang Chen