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  • KD/KO Validated

PHLDA1 Polyclonal antibody

PHLDA1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 18263-1-AP

Synonyms

DT1P1B11, PHLDA1, PHRIP, PQ rich protein, PQR protein, TDAG51



Tested Applications

Positive WB detected inA375 cells, mouse brain tissue, rat heart tissue, BXPC-3 cells, HeLa cells, MDA-MB-231 cells
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18263-1-AP is PHLDA1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHLDA1 fusion protein Ag13125
Full Name pleckstrin homology-like domain, family A, member 1
Calculated molecular weight 45 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC018929
Gene symbol PHLDA1
Gene ID (NCBI) 22822
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PHLDA1 antibody 18263-1-APDownload protocol
IF protocol for PHLDA1 antibody 18263-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Ther Nucleic Acids

lncRNA HIF1A Antisense RNA 2 Modulates Trophoblast Cell Invasion and Proliferation through Upregulating PHLDA1 Expression.

Authors - Dan Wu
humanWB,IHC,IF

Cell Death Dis

The long non-coding RNA SNHG1 promotes glioma progression by competitively binding to miR-194 to regulate PHLDA1 expression.

Authors - Liang Liu
  • KD Validated
mouseWB

Hum Exp Toxicol

Loss of PHLDA1 has a protective role in OGD/R-injured neurons via regulation of the GSK-3β/Nrf2 pathway.

Authors - F Yang
  • KD Validated
ratWB

Life Sci

PHLDA1 is a new therapeutic target of oxidative stress and ischemia reperfusion-induced myocardial injury.

Authors - Yuxuan Guo
  • KD Validated
mouseWB

Brain Behav Immun

PHLDA1 promotes microglia-mediated neuroinflammation via regulating K63-linked ubiquitination of TRAF6.

Authors - Chaojun Han
  • KD Validated