PHOX2B Monoclonal antibody

PHOX2B Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1F5F5

Cat no : 66254-1-Ig

Synonyms

NBPhox, Neuroblastoma Phox, paired like homeobox 2b, PHOX2B, PHOX2B homeodomain protein, PMX2B



Tested Applications

Positive WB detected inNeuro-2a cells
Positive IHC detected inneuroblastoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

66254-1-Ig targets PHOX2B in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PHOX2B fusion protein Ag13196
Full Name paired-like homeobox 2b
Calculated molecular weight 31 kDa
Observed molecular weight 32-36 kDa
GenBank accession numberBC017199
Gene symbol PHOX2B
Gene ID (NCBI) 8929
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PHOX2B antibody 66254-1-IgDownload protocol
IHC protocol for PHOX2B antibody 66254-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Sci Signal

Mitotic cell responses to substrate topological cues are independent of the molecular nature of adhesion.

Authors - Ouranio Anastasiou