|Positive WB detected in||K-562 cells, HL-60 cells, mouse ovary tissue, mouse pancreas tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
14242-1-AP targets PIAS4 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PIAS4 fusion protein Ag5498|
|Full Name||protein inhibitor of activated STAT, 4|
|Calculated molecular weight||57 kDa|
|Observed molecular weight||57-70 kDa|
|GenBank accession number||BC029874|
|Gene ID (NCBI)||51588|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PIAS4(protein inhibitor of activated STAT protein 4) is also named as PIASG, PIASy and belongs to the PIAS family. It enhanced the conjugation of SUMO2 to GATA2 through its E3 SUMO ligase activity. TGF-b can up-regulates PLAS4 expression in MCs to down-regulatea-SMA gene transcription by the interaction with E12 and PLAS4 predominantly acts as E3 ligases for E12 in MCs among PIAS family members (PMID:22829926). This protein can be sumoylated and Lys-35 is the main site of sumoylation(PMID:15831457).
DPPA2/4 and SUMO E3 ligase PIAS4 opposingly regulate zygotic transcriptional program.
Protein inhibitor of activated STAT, PIASy regulates α-smooth muscle actin expression by interacting with E12 in mesangial cells.
Rad18-dependent SUMOylation of human specialized DNA polymerase eta is required to prevent under-replicated DNA.