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  • KD/KO Validated

PIAS4 Polyclonal antibody

PIAS4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 14242-1-AP

Synonyms

E3 SUMO protein ligase PIAS4, PIAS gamma, PIAS4, Piasg, PIASY, ZMIZ6



Tested Applications

Positive WB detected inK-562 cells, HL-60 cells, mouse ovary tissue, mouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

14242-1-AP targets PIAS4 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIAS4 fusion protein Ag5498
Full Name protein inhibitor of activated STAT, 4
Calculated molecular weight 57 kDa
Observed molecular weight 57-70 kDa
GenBank accession numberBC029874
Gene symbol PIAS4
Gene ID (NCBI) 51588
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PIAS4(protein inhibitor of activated STAT protein 4) is also named as PIASG, PIASy and belongs to the PIAS family. It enhanced the conjugation of SUMO2 to GATA2 through its E3 SUMO ligase activity. TGF-b can up-regulates PLAS4 expression in MCs to down-regulatea-SMA gene transcription by the interaction with E12 and PLAS4 predominantly acts as E3 ligases for E12 in MCs among PIAS family members (PMID:22829926). This protein can be sumoylated and Lys-35 is the main site of sumoylation(PMID:15831457).

Protocols

Product Specific Protocols
WB protocol for PIAS4 antibody 14242-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

PLoS Biol

DPPA2/4 and SUMO E3 ligase PIAS4 opposingly regulate zygotic transcriptional program.

Authors - Yao-Long Yan
  • KD Validated
mouseWB

PLoS One

Protein inhibitor of activated STAT, PIASy regulates α-smooth muscle actin expression by interacting with E12 in mesangial cells.

Authors - Torikoshi Kazuo K
  • KD Validated
humanWB

Nat Commun

Rad18-dependent SUMOylation of human specialized DNA polymerase eta is required to prevent under-replicated DNA.

Authors - Emmanuelle Despras
  • KD Validated