Tested Applications
| Positive WB detected in | HeLa cells, mouse testis tissue, mouse lung tissue, Jurkat cells |
| Positive IHC detected in | mouse skeletal muscle tissue, human stomach cancer tissue, mouse skeletal muscle tissue, mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13723-1-AP targets VPS34 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | phosphoinositide-3-kinase, class 3 |
| Calculated Molecular Weight | 887 aa, 100 kDa |
| Observed Molecular Weight | 102 kDa |
| GenBank Accession Number | BC053651 |
| Gene Symbol | VPS34 |
| Gene ID (NCBI) | 5289 |
| RRID | AB_10646474 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NEB9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PIK3C3(Phosphatidylinositol 3-kinase catalytic subunit type 3) is also named as VPS34 and belongs to the PI3/PI4-kinase family.It is a catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes.It can form a dimer(PMID:20339072).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for VPS34 antibody 13723-1-AP | Download protocol |
| WB protocol for VPS34 antibody 13723-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Beclin-1 Antibody (Cat# 13723-1-AP) has 6 citations published in journals including Nature Structural & Molecular Biology, Cancer Letters, Journal of Virology, Frontiers in Pharmacology, Psychopharmacology, and Journal of Cardiovascular Translational Research. Research fields span autophagy regulation, bladder cancer metastasis, aging-related autophagy decline, RSV-induced lung inflammation, coronavirus entry mechanisms, ADHD cognitive dysfunction, and myocardial ischemia-reperfusion injury.
| Species | Application | Title |
|---|---|---|
Cancer Lett Nutrient deprivation promotes bladder cancer metastasis through Beclin-1 deacetylation-mediated autophagy activation. | ||
Nat Struct Mol Biol Reduction of DHHC5-mediated beclin 1 S-palmitoylation underlies autophagy decline in aging | ||
Front Pharmacol Integrated Network Pharmacology and Lipidomics to Reveal the Inhibitory Effect of Qingfei Oral Liquid on Excessive Autophagy in RSV-Induced Lung Inflammation. | ||
J Virol Potassium molybdate blocks APN-dependent coronavirus entry by degrading receptor via PIK3C3-mediated autophagy
| ||
Psychopharmacology (Berl) 3-Methyladenine administration alleviates cognitive and memory dysfunction in attention-deficit/hyperactivity disorder by modulating autophagy. | ||
J Cardiovasc Transl Res The Protective Mechanism of Dexmedetomidine in Regulating Atg14L-Beclin1-Vps34 Complex Against Myocardial Ischemia-Reperfusion Injury. |
