VPS15 Polyclonal antibody

VPS15 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17894-1-AP

Publications (13)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA
For FC, select conjugated version.

Synonyms

PI3K p150, PIK3R4, EC:2.7.11.1, p150, Phosphoinositide 3-kinase adaptor protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

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Filter: AllWBIHCIF/ICC

WB analysis of HepG2 using 17894-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17894-1-AP (VPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 17894-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17894-1-AP (VPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 17894-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17894-1-AP (VPS15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human appendicitis using 17894-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17894-1-AP (VPS15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 17894-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17894-1-AP (VPS15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse pancreas using 17894-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17894-1-AP (VPS15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse spleen using 17894-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17894-1-AP (VPS15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat kidney using 17894-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17894-1-AP (VPS15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat spleen using 17894-1-AP
Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 17894-1-AP (VPS15 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 17894-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17894-1-AP (VPS15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat kidney using 17894-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17894-1-AP (VPS15 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 17894-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VPS15 antibody (17894-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (13)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, HeLa cells
Positive IHC detected in	human appendicitis tissue, rat spleen tissue, mouse kidney tissue, mouse pancreas tissue, rat kidney tissue, mouse spleen tissue, human ovary cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17894-1-AP targets VPS15 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12221 Product name: Recombinant human PIK3R4 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-330 aa of BC110318 Sequence: MGNQLAGIAPSQILSVESYFSDIHDFEYDKSLGSTRFFKVARAKHREGLVVVKVFAIQDPTLPLTSYKQELEELKIRLNSAQNCLPFQKASEKASEKAAMLFRQYVRDNLYDRISTRPFLNNIEKRWIAFQILTAVDQAHKSGVRHGDIKTENVMVTSWNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDGGMFATELEYMRDPSTPLVDLNSNQRTRGELKRAMDIFSAGCVIAELFTEGVPLFDLSQLLAYRNGHFFPEQVLNKIEDHSIRELVTQMIHREPDKRLEAEDYLKQQRGNAFPEIFYTFLQPYMAQ Predict reactive species
Full Name	phosphoinositide-3-kinase, regulatory subunit 4
Calculated Molecular Weight	1358 aa, 153 kDa
Observed Molecular Weight	150 kDa
GenBank Accession Number	BC110318
Gene Symbol	PIK3R4
Gene ID (NCBI)	30849
RRID	AB_2163630
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q99570
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIK3R4(phosphoinositide 3-kinase regulatory subunit 4) is also named as PI3-kinase p150 subunit and belongs to the Ser/Thr protein kinase family. It is a regulatory subunit of the PI3K complex and may regulate membrane trafficking late in the endocytic pathway. This protein can be probably autophosphorylated(PMID:19369195).

Protocols

Product Specific Protocols
IF protocol for VPS15 antibody 17894-1-AP	Download protocol
IHC protocol for VPS15 antibody 17894-1-AP	Download protocol
WB protocol for VPS15 antibody 17894-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The VPS15 Polyclonal antibody (Cat# 17894-1-AP) has 13 total citations. These appear in journals including Nature Neuroscience, Nature Communications, Autophagy, Nature Structural & Molecular Biology, Developmental Cell, Science Bulletin, Cancer Letters, Journal of Hazardous Materials, Theriogenology, and Experimental & Therapeutic Medicine. Research fields span autophagy regulation, neurodevelopment, cancer biology, membrane trafficking, aging, reproductive biology, and cardiovascular protection.

Species	Application	Title
mouse,human	WB,IF	Sci Bull (Beijing) A germline-specific vesicular structure licenses mRNA activation during spermiogenesis and is hijacked in gastric cancer. Authors - Mingxi Liu View Article
mouse	WB	Nat Neurosci Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans. Authors - Thomas Gstrein View Article
	WB	Autophagy HSPA8 regulates anti-bacterial autophagy through liquid-liquid phase separation Authors - Chunhui Miao View Article
mouse	WB	Autophagy MLKL-USP7-UBA52 signaling is indispensable for autophagy in brain through maintaining ubiquitin homeostasis Authors - Zhigang Zhang View Article
human	WB	Nat Commun Structural basis for VPS34 kinase activation by Rab1 and Rab5 on membranes. Authors - Shirley Tremel View Article
	IP,CoIP	Nat Commun IL-6 regulates autophagy and chemotherapy resistance by promoting BECN1 phosphorylation. Authors - Fuqing Hu View Article