VPS15 Polyclonal antibody

VPS15 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 17894-1-AP

Synonyms

p150, PI3 kinase p150 subunit, PI3K p150, PI3-kinase regulatory subunit 4, PIK3R4, VPS15



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

17894-1-AP targets VPS15 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS15 fusion protein Ag12221
Full Name phosphoinositide-3-kinase, regulatory subunit 4
Calculated molecular weight 1358 aa, 153 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC110318
Gene symbol PIK3R4
Gene ID (NCBI) 30849
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PIK3R4(phosphoinositide 3-kinase regulatory subunit 4) is also named as PI3-kinase p150 subunit and belongs to the Ser/Thr protein kinase family. It is a regulatory subunit of the PI3K complex and may regulate membrane trafficking late in the endocytic pathway. This protein can be probably autophosphorylated(PMID:19369195).

Protocols

Product Specific Protocols
WB protocol for VPS15 antibody 17894-1-APDownload protocol
IF protocol for VPS15 antibody 17894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Exp Ther Med

Protective effects of p-nitro caffeic acid phenethyl ester on acute myocardial ischemia-reperfusion injury in rats.

Authors - Qin DU

Cancer Lett

Targeting AR-Beclin 1 Complex-Modulated Growth Factor Signaling Increases the Antiandrogen-Enzalutamide Sensitivity to Better Suppress the Castration-Resistant Prostate Cancer Growth.

Authors - Meng Zhang
mouseWB

Nat Neurosci

Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans.

Authors - Thomas Gstrein