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PIP4K2A Polyclonal antibody

PIP4K2A Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12469-1-AP

Synonyms

PI(5)P 4 kinase type II alpha, PI5P4KA, PIP4K2A, PIP4KII alpha, PIP5K2, PIP5K2A, PIP5KII alpha, PIP5KIIA, PIP5KIII, PIPK, PtdIns(4)P 5 kinase B isoform, PtdIns(4)P 5 kinase C isoform

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PIP4K2A Polyclonal antibody (12469-1-AP)

    WB analysis of mouse brain using 12469-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PIP4K2A Polyclonal antibody (12469-1-AP)

    WB analysis using 12469-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PIP4K2A Polyclonal antibody (12469-1-AP)

    WB analysis of mouse brain using 12469-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PIP4K2A Polyclonal antibody (12469-1-AP)

    WB analysis using 12469-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PIP4K2A Polyclonal antibody (12469-1-AP)

    IP experiment of mouse brain using 12469-1-AP

    IP result of anti-PIP4K2A (IP:12469-1-AP, 4ug; Detection:12469-1-AP 1:500) with mouse brain tissue lysate 4400ug.

  • Immunohistochemistry (IHC) staining of human spleen tissue using PIP4K2A Polyclonal antibody (12469-1-AP)

    IHC staining of human spleen using 12469-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12469-1-AP (PIP4K2A Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PIP4K2A Polyclonal antibody (12469-1-AP)

    IHC staining of human spleen using 12469-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12469-1-AP (PIP4K2A Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PIP4K2A Polyclonal antibody (12469-1-AP)

    IHC staining of mouse brain using 12469-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12469-1-AP (PIP4K2A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PIP4K2A Polyclonal antibody (12469-1-AP)

    IHC staining of mouse brain using 12469-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12469-1-AP (PIP4K2A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using PIP4K2A Polyclonal antibody (12469-1-AP)

    IF Staining of SH-SY5Y using 12469-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 12469-1-AP (PIP4K2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PIP4K2A Polyclonal antibody (12469-1-AP)

    IF Staining of HepG2 using 12469-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PIP4K2A antibody (12469-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (black).

Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman spleen tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12469-1-AP targets PIP4K2A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIP4K2A fusion protein Ag3152
Full Name phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
Calculated Molecular Weight 406 aa, 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC018034
Gene Symbol PIP4K2A
Gene ID (NCBI) 5305
RRIDAB_10639518
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PIP4K2A antibody 12469-1-APDownload protocol
IHC protocol for PIP4K2A antibody 12469-1-APDownload protocol
IF protocol for PIP4K2A antibody 12469-1-APDownload protocol
IP protocol for PIP4K2A antibody 12469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanIF,IHC

Dev Cell

PI5P4Ks drive metabolic homeostasis through peroxisome-mitochondria interplay.

Authors - Archna Ravi
View Article
humanIF,IHC,IP,WB

Int J Biochem Cell Biol

A novel lncRNA lncSAMD11-1:1 interacts with PIP4K2A to promote endometrial decidualization by stabilizing FoxO1 nuclear localization

Authors - Mei Zhang
View Article
human,mouseWB,IHC,IF

Sci Adv

PI5P4Kα supports prostate cancer metabolism and exposes a survival vulnerability during androgen receptor inhibition

Authors - Joanna Triscott
  • KD Validated
View Article